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. 1985 Aug;55(2):509–512. doi: 10.1128/jvi.55.2.509-512.1985

Diagnosis of rotavirus infection with cloned cDNA copies of viral genome segments.

M Lin, M Imai, A R Bellamy, N Ikegami, Y Furuichi, D Summers, D L Nuss, R Deibel
PMCID: PMC254963  PMID: 2991576

Abstract

The diagnostic potential of cloned cDNA copies of human rotavirus (strain WA) genome segments for the detection of rotavirus in clinical specimens has been determined. A hybridization assay in which a mixture of 32P-labeled cDNAs representing the 11 rotavirus segments was used as a probe compared favorably with three frequently used diagnostic tests for rotavirus in terms of both specificity and sensitivity. Significantly, clinical isolates could be readily distinguished when cloned cDNA copies of individual genome segments were used independently as a probe. In assays in which genome RNA from rotaviruses of known subgroups and serotypes were tested, cloned probes that encode nonstructural viral proteins hybridized efficiently to genome RNAs of all strains, whereas cloned probes corresponding to genome segments 6 and 9 exhibited the potential for differentiating strains of different subgroups and serotypes. Cloned cDNA copies of rotavirus genome segments therefore offer considerable potential for improved general diagnosis of rotavirus in clinical specimens, as well as for epidemiological studies in which virus isolates can be distinguished on the basis of nucleotide sequence homology of individual genome segments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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