Abstract
The cohesive single-stranded ends of temperate Bacillus subtilis phage phi 105 were analyzed with the exonuclease activities of the Klenow fragment of DNA polymerase I and with exonuclease III and were found to be 3' extensions. Chemical sequencing of 3'-end-labeled fragments showed that the ends are 7-base extended 3' single strands and have the sequence: 5'-GCGCTCC-3'. 3'-CGCGAGG-5'
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- Armentrout R. W., Rutberg L. Mapping of prophage and mature deoxyribonucleic acid from temperate Bacillus bacteriophage phi 105 by marker rescue. J Virol. 1970 Dec;6(6):760–767. doi: 10.1128/jvi.6.6.760-767.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Armentrout R. W., Skoog L., Rutberg L. Structure and biological activity of deoxyribonucleic acid from Bacillus bacteriophage phi 105: effects of Escherichia coli exonucleases. J Virol. 1971 Mar;7(3):359–371. doi: 10.1128/jvi.7.3.359-371.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chow L. T., Boice L., Davidson N. Map of the partial sequence homology between DNA molecules of Bacillus subtilis bacteriophages SPO2 and phi105. J Mol Biol. 1972 Jul 28;68(3):391–400. doi: 10.1016/0022-2836(72)90093-9. [DOI] [PubMed] [Google Scholar]
- Chow L. T., Davidson N. Electron microscope study of the structures of the Bacillus subtilis prophages, SPO2 and phi105. J Mol Biol. 1973 Apr 5;75(2):257–264. doi: 10.1016/0022-2836(73)90019-3. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Scher B. M., Dean D. H., Garro A. J. Fragmentation of Bacillus bacteriophage phi105 DNA by complementary single-stranded DNA in the cohesive ends of the molecule. J Virol. 1977 Aug;23(2):377–383. doi: 10.1128/jvi.23.2.377-383.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shapiro J. A., Dean D. H., Halvorson H. O. Low-frequency specialized transduction with Bacillus subtilis bacteriophage phi 105. Virology. 1974 Dec;62(2):393–403. doi: 10.1016/0042-6822(74)90401-2. [DOI] [PubMed] [Google Scholar]
- Wu R., Taylor E. Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. J Mol Biol. 1971 May 14;57(3):491–511. doi: 10.1016/0022-2836(71)90105-7. [DOI] [PubMed] [Google Scholar]