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. 1985 Jan;53(1):273–278. doi: 10.1128/jvi.53.1.273-278.1985

Spontaneous expression of C-type virus in DBA/2 mice is associated with an increased rate of mortality, independent of neoplastic disease.

K Ulrich, B A Nexø
PMCID: PMC255029  PMID: 2981347

Abstract

Ecotropic C-type retroviruses isolated from both normal and dimethylbenzanthracene-treated DBA/2 mice could be classified into three major groups, Ea, Eb, and Ec, that differed in structure and biology. Weanling DBA/2 mice were generally free of viruses, but a fraction of adult individuals became virus positive and were apparently selectively associated with a high expression of the Eb viruses. Some of the ecotropic viruses from DBA/2 mice acted as exogenous pathogens. They caused viremia and a moderate incidence of leukemia when injected into C3H and ST/a mice. In addition, they caused an appreciable number of early deaths without signs of malignancy. To evaluate the natural role of the viruses, we studied the survival of spontaneously viremic and nonviremic DBA/2 mice. The viremic animals as a group were characterized by a significantly reduced life-span that was not related to neoplasia. These observations indicated that endogenous C-type retroviruses can be pathogenic without preselection of the host for disease. They also emphasize that endogenous viruses, like their exogenous counterparts, can have a broader pathogenic spectrum than normally appreciated.

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Selected References

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  1. Aoki T., Boyse E. A., Old L. J. Wild-type Gross leukemia virus. I. Soluble antigen (GSA) in the plasma and tissues of infected mice. J Natl Cancer Inst. 1968 Jul;41(1):89–96. [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Chattopadhyay S. K., Cloyd M. W., Linemeyer D. L., Lander M. R., Rands E., Lowy D. R. Cellular origin and role of mink cell focus-forming viruses in murine thymic lymphomas. Nature. 1982 Jan 7;295(5844):25–31. doi: 10.1038/295025a0. [DOI] [PubMed] [Google Scholar]
  4. Cloyd M. W., Hartley J. W., Rowe W. P. Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1980 Mar 1;151(3):542–552. doi: 10.1084/jem.151.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crittenden L. B., Witter R. L., Okazaki W., Neiman P. E. Lymphoid neoplasms in chicken flocks free of infection with exogenous avian tumor viruses. J Natl Cancer Inst. 1979 Jul;63(1):191–200. [PubMed] [Google Scholar]
  6. Cullen S. E., Schwartz B. D. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci. J Immunol. 1976 Jul;117(1):136–142. [PubMed] [Google Scholar]
  7. De Rossi A., D'Andrea E., Colombatti A., Fischinger P. J., Chieco-Bianchi L. Spontaneous lymphomas in SJL/J-(v+) mice: ecotropic and dualtropic virus expression in normal and lymphomatous tissues. J Natl Cancer Inst. 1981 Dec;67(6):1241–1250. [PubMed] [Google Scholar]
  8. GROSS L. "Spontaneous" leukemia developing in C3H mice following inoculation in infancy, with AK-leukemic extracts, or AK-embrvos. Proc Soc Exp Biol Med. 1951 Jan;76(1):27–32. [PubMed] [Google Scholar]
  9. Gardner M. B., Henderson B. E., Estes J. D., Rongey R. W., Casagrande J., Pike M., Huebner R. J. The epidemiology and virology of C-type virus-associated hematological cancers and related diseases in wild mice. Cancer Res. 1976 Feb;36(2 Pt 2):574–581. [PubMed] [Google Scholar]
  10. Gisselbrecht S., Pozo F., Debré P., Hurot M. A., Lacombe M. J., Levy J. P. Genetic control of sensitivity to Moloney-virus-induced leukemias in mice. I. Demonstration of multigenic control. Int J Cancer. 1978 May 15;21(5):626–634. doi: 10.1002/ijc.2910210513. [DOI] [PubMed] [Google Scholar]
  11. Greenberger J. S., Stephenson J. R., Moloney W. C., Aaronson S. A. Different hematological diseases induced by type C viruses chemically activated from embryo cells of different mouse strains. Cancer Res. 1975 Jan;35(1):245–252. [PubMed] [Google Scholar]
  12. Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
  13. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hartley J. W., Yetter R. A., Morse H. C., 3rd A mouse gene on chromosome 5 that restricts infectivity of mink cell focus-forming recombinant murine leukemia viruses. J Exp Med. 1983 Jul 1;158(1):16–24. doi: 10.1084/jem.158.1.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Henderson I. C., Lieber M. M., Todaro G. J. Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. Virology. 1974 Jul;60(1):282–287. doi: 10.1016/0042-6822(74)90386-9. [DOI] [PubMed] [Google Scholar]
  16. LEERHOY J. CYTOPATHIC EFFECT OF RUBELLA VIRUS IN A RABBIT-CORNEA CELL LINE. Science. 1965 Aug 6;149(3684):633–634. doi: 10.1126/science.149.3684.633. [DOI] [PubMed] [Google Scholar]
  17. Lilly F., Duran-Reynals M. L., Rowe W. P. Correlation of early murine leukemia virus titer and H-2 type with spontaneous leukemia in mice of the BALB/c times AKR cross: a genetic analysis. J Exp Med. 1975 Apr 1;141(4):882–889. [PMC free article] [PubMed] [Google Scholar]
  18. Meier H., Taylor B. A., Cherry M., Buebner R. J. Host-gene control of type-C RNA tumor virus expression and tumorigenesis in inbred mice. Proc Natl Acad Sci U S A. 1973 May;70(5):1450–1455. doi: 10.1073/pnas.70.5.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Motta J. V., Crittenden L. B., Purchase H. G., Stone H. A., Witter R. L. Low oncogenic potential of avian endogenous RNA tumor virus infection or expression. J Natl Cancer Inst. 1975 Sep;55(3):685–689. doi: 10.1093/jnci/55.3.685. [DOI] [PubMed] [Google Scholar]
  20. Nexo B. A. A plaque assay for murine leukemia virus using enzyme-coupled antibodies. Virology. 1977 Apr;77(2):849–852. doi: 10.1016/0042-6822(77)90504-9. [DOI] [PubMed] [Google Scholar]
  21. Nexø B. A., Krog H. H. C-type virus protein p30 in blood from inbred mice correlates with their later incidence of leukemia. Infect Immun. 1977 Feb;15(2):376–381. doi: 10.1128/iai.15.2.376-381.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nexø B. A., Ulrich K. Activation of C-type virus during chemically induced leukemogenesis in mice. Cancer Res. 1978 Mar;38(3):729–735. [PubMed] [Google Scholar]
  23. Nexø B. A., Ulrich K. Variants of type-C retroviruses from DBA/2 mice: protein-structural and biological properties. Virology. 1983 Mar;125(2):454–467. doi: 10.1016/0042-6822(83)90216-7. [DOI] [PubMed] [Google Scholar]
  24. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  25. Quint W., Quax W., van der Putten H., Berns A. Characterization of AKR murine leukemia virus sequences in AKR mouse substrains and structure of integrated recombinant genomes in tumor tissues. J Virol. 1981 Jul;39(1):1–10. doi: 10.1128/jvi.39.1.1-10.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ruscetti S., Davis L., Feild J., Oliff A. Friend murine leukemia virus-induced leukemia is associated with the formation of mink cell focus-inducing viruses and is blocked in mice expressing endogenous mink cell focus-inducing xenotropic viral envelope genes. J Exp Med. 1981 Sep 1;154(3):907–920. doi: 10.1084/jem.154.3.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schwarz H., Ihle J. N., Wecker E., Fischinger P. J., Thiel H. J., Bolognesi D. P., Schäfer W. Properties of mouse leukemia viruses. XVII. Factors required for successful treatment of spontaneous AKR leukemia by antibodies against gp71. Virology. 1981 Jun;111(2):568–578. doi: 10.1016/0042-6822(81)90358-5. [DOI] [PubMed] [Google Scholar]
  28. Ulrich K., Nexo J. A. Virus protein p30 in blood predicts development of leukaemia in mice injected with MuLV. Nature. 1977 Jun 23;267(5613):723–724. doi: 10.1038/267723a0. [DOI] [PubMed] [Google Scholar]
  29. Willumsen B. M. In vitro selection of high-infectious, leukemogenic virus from low-infectious, non-leukemogenic type C virus from a malignant ST/a mouse cell line. J Virol. 1979 Mar;29(3):1213–1220. doi: 10.1128/jvi.29.3.1213-1220.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. van der Putten H., Quint W., van Raaij J., Maandag E. R., Verma I. M., Berns A. M-MuLV-induced leukemogenesis: integration and structure of recombinant proviruses in tumors. Cell. 1981 Jun;24(3):729–739. doi: 10.1016/0092-8674(81)90099-4. [DOI] [PubMed] [Google Scholar]

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