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. 1985 Jan;53(1):324–329. doi: 10.1128/jvi.53.1.324-329.1985

RNase H-mediated release of the retrovirus RNA polyadenylate tail during reverse transcription.

J C Olsen, K F Watson
PMCID: PMC255046  PMID: 2578196

Abstract

By examining enzymatic reactions in vitro, we found that an early event during reverse transcription of the avian myeloblastosis virus RNA genome is the release of the 3' polyadenylate tail from the viral RNA template. By using specially constructed molecules containing minus-strand, strong-stop DNA and the 3' end of the viral RNA genome, we found that the reverse transcriptase-associated RNase H is responsible for the endonucleolytic release of polyadenylate.

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Selected References

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