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. 1985 Sep;55(3):736–743. doi: 10.1128/jvi.55.3.736-743.1985

Interaction between various polymerized human albumins and hepatitis B surface antigen.

M W Yu, J S Finlayson, J W Shih
PMCID: PMC255057  PMID: 4020964

Abstract

A variety of albumin polymers were prepared and tested for binding with hepatitis B surface antigen (HBsAg): synthetic polymers cross-linked by either glutaraldehyde or carbodiimide; heat-aggregated polymers made by heating albumin solutions at 60 degrees C for 10 h with or without albumin stabilizer; and polymers isolated from fresh or long-stored commercial therapeutic albumin solutions. A sensitive solid-phase, competitive-inhibition radioimmunoassay, which can detect as little as 10 ng of glutaraldehyde-cross-linked human albumin polymer (PHALB-G), was developed and used to measure binding. The binding of PHALB-G with HBsAg was 150- to 1,000-fold greater than that of any other albumin polymer. Glutaraldehyde-cross-linked bovine albumin polymer showed no binding. Albumin monomer and dimer fractions produced by glutaraldehyde treatment exhibited some binding, albeit much weaker than PHALB-G. As measured by a direct-binding assay with solid-phase PHALB-G, the attachment of HBsAg particles from sera positive for antibody to the e antigen was less efficient than that from sera positive for e antigen, even when all sera were tested at equal HBsAg concentrations. In protein blot experiments with radiolabeled albumin preparations, PHALB-G bound almost exclusively to HBsAg polypeptide P31 and showed no binding with the major polypeptides P23 and P26. None of the other radiolabeled albumin polymers was reactive. These results indicate that the interaction between PHALB-G and HBsAg is not due to polymerization of albumin per se, but rather is unique and site specific.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Gerin J. L., Faust R. M., Holland P. V. Biophysical characterization of the adr subtype of hepatitis B antigen and preparation of anti-r sera in rabbits. J Immunol. 1975 Jul;115(1):100–105. [PubMed] [Google Scholar]
  3. Habeeb A. J., Hiramoto R. Reaction of proteins with glutaraldehyde. Arch Biochem Biophys. 1968 Jul;126(1):16–26. doi: 10.1016/0003-9861(68)90554-7. [DOI] [PubMed] [Google Scholar]
  4. Hansson B. G., Purcell R. H. Sites that bind polymerized albumin on hepatitis B surface antigen particles: detection by radioimmunoassay. Infect Immun. 1979 Oct;26(1):125–130. doi: 10.1128/iai.26.1.125-130.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Imai M., Yanase Y., Nojiri T., Miyakawa Y., Mayumi M. A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology. 1979 Feb;76(2):242–247. [PubMed] [Google Scholar]
  6. Ionescu-Matiu I., Sanchez Y., Hollinger F. B., Melnick J. L., Dreesman G. R. Presence of a receptor for albumin on individual HBsAg polypeptides and on HBsAg produced by a hepatoma cell line. J Med Virol. 1980;6(2):175–178. doi: 10.1002/jmv.1890060211. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Lenkei R., Babes V. T., Dan M. E., Mustea A., Dobre I. Correlations between anti-albumin antibodies and HBsAG in hepatic patients. J Med Virol. 1977;1(1):29–34. doi: 10.1002/jmv.1890010106. [DOI] [PubMed] [Google Scholar]
  9. Lenkei R., Ghetie V. Methods for detection of anti-albumin autoantibodies in hepatic diseases. J Immunol Methods. 1977;16(1):23–30. doi: 10.1016/0022-1759(77)90036-9. [DOI] [PubMed] [Google Scholar]
  10. Lenkei R., Onica D., Ghetie V. Receptors for polymerized albumin on liver cells. Experientia. 1977 Aug 15;33(8):1046–1047. doi: 10.1007/BF01945961. [DOI] [PubMed] [Google Scholar]
  11. Machida A., Kishimoto S., Ohnuma H., Miyamoto H., Baba K., Oda K., Nakamura T., Miyakawa Y., Mayumi M. A hepatitis B surface antigen polypeptide (P31) with the receptor for polymerized human as well as chimpanzee albumins. Gastroenterology. 1983 Aug;85(2):268–274. [PubMed] [Google Scholar]
  12. Milich D. R., Gottfried T. D., Vyas G. N. Characterization of the interaction between polymerized human albumin and hepatitis B surface antigen. Gastroenterology. 1981 Aug;81(2):218–225. [PubMed] [Google Scholar]
  13. Neurath A. R., Kent S. B., Strick N. Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus. Science. 1984 Apr 27;224(4647):392–395. doi: 10.1126/science.6200931. [DOI] [PubMed] [Google Scholar]
  14. Neurath A. R., Strick N. Radioimmunoassay for albumin-binding sites associated with HBsAg: correlation of results with the presence of e-antigen in serum. Intervirology. 1979;11(2):128–132. doi: 10.1159/000149024. [DOI] [PubMed] [Google Scholar]
  15. O'Neill S. P. Interaction of hepatitis B surface antigen with polymerized human serum albumin. J Med Virol. 1979;4(3):177–185. doi: 10.1002/jmv.1890040304. [DOI] [PubMed] [Google Scholar]
  16. Onica D., Dobre M. A., Lenkei R. Immunogenicity of glutaraldehyde treated homologous monomeric albumin in rabbits. Immunochemistry. 1978 Dec;15(12):941–944. doi: 10.1016/0161-5890(78)90128-1. [DOI] [PubMed] [Google Scholar]
  17. Onică D., Lenkei R., Gheţie V. Immunogenicity of glutaraldehyde-treated homologous albumin in rabbits. Immunochemistry. 1978 Sep;15(9):687–693. doi: 10.1016/0161-5890(78)90043-3. [DOI] [PubMed] [Google Scholar]
  18. Onică D., Mărgineanu I., Medeşan C., Călugăru A., Manciulea M. Antibodies against glutaraldehyde-modified albumin in normal mouse sera. Mol Immunol. 1982 Aug;19(8):1021–1027. doi: 10.1016/0161-5890(82)90310-8. [DOI] [PubMed] [Google Scholar]
  19. Pontisso P., Alberti A., Schiavon E., Tremolada F., Bortolotti F., Realdi G. Receptors for polymerized human serum albumin on hepatitis B virus particles detected by radioimmunoassay: changes in receptor activity in serum during acute and chronic infection. J Virol Methods. 1983 Mar;6(3):151–159. doi: 10.1016/0166-0934(83)90027-7. [DOI] [PubMed] [Google Scholar]
  20. Stibbe W., Gerlich W. H. Structural relationships between minor and major proteins of hepatitis B surface antigen. J Virol. 1983 May;46(2):626–628. doi: 10.1128/jvi.46.2.626-628.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thung S. N., Gerber M. A. Albumin binding sites of human hepatocytes. Liver. 1983 Oct;3(5):290–294. doi: 10.1111/j.1600-0676.1983.tb00880.x. [DOI] [PubMed] [Google Scholar]
  22. Thung S. N., Gerber M. A. Polyalbumin receptors: their role in the attachment of hepatitis B virus to hepatocytes. Semin Liver Dis. 1984 Feb;4(1):69–75. doi: 10.1055/s-2008-1040647. [DOI] [PubMed] [Google Scholar]
  23. Thung S. N., Gerber M. A. Specificities of albumin receptors and albumin antibodies. Infect Immun. 1981 Jun;32(3):1292–1294. doi: 10.1128/iai.32.3.1292-1294.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Trevisan A., Gudat F., Guggenheim R., Krey G., Dürmüller U., Lüond G., Düggelin M., Landmann J., Tondelli P., Bianchi L. Demonstration of albumin receptors on isolated human hepatocytes by light and scanning electron microscopy. Hepatology. 1982 Nov-Dec;2(6):832–835. doi: 10.1002/hep.1840020616. [DOI] [PubMed] [Google Scholar]
  26. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  27. Yu M. W., Finlayson J. S. Quantitative determination of the stabilizers octanoic acid and N-acetyl-DL-tryptophan in human albumin products. J Pharm Sci. 1984 Jan;73(1):82–86. doi: 10.1002/jps.2600730122. [DOI] [PubMed] [Google Scholar]
  28. Yu M. W., Finlayson J. S. Stabilization of human albumin by caprylate and acetyltryptophanate. Vox Sang. 1984;47(1):28–40. doi: 10.1111/j.1423-0410.1984.tb01558.x. [DOI] [PubMed] [Google Scholar]

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