Abstract
Wild-type BK virus, which has three 68-base-pair (bp) elements tandemly repeated in its transcriptional control region, forms clear, large plaques in human embryonic kidney cell cultures but rarely transforms hamster or rat cells. From this BK virus DNA, deletion mutants with fewer than three 68-bp elements were constructed and characterized. The mutant DNA with two 68-bp elements formed small, turbid plaques in human cell cultures and transformed rat 3Y1 cells inefficiently. The mutant DNA with one 68-bp element formed minute, turbid plaques in human cell cultures and transformed rat cells efficiently.
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- Byrne B. J., Davis M. S., Yamaguchi J., Bergsma D. J., Subramanian K. N. Definition of the simian virus 40 early promoter region and demonstration of a host range bias in the enhancement effect of the simian virus 40 72-base-pair repeat. Proc Natl Acad Sci U S A. 1983 Feb;80(3):721–725. doi: 10.1073/pnas.80.3.721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
- Hearing P., Shenk T. The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element. Cell. 1983 Jul;33(3):695–703. doi: 10.1016/0092-8674(83)90012-0. [DOI] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- McCutchan J. H., Pagano J. S. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran. J Natl Cancer Inst. 1968 Aug;41(2):351–357. [PubMed] [Google Scholar]
- Rosenthal N., Kress M., Gruss P., Khoury G. BK viral enhancer element and a human cellular homolog. Science. 1983 Nov 18;222(4625):749–755. doi: 10.1126/science.6314501. [DOI] [PubMed] [Google Scholar]
- Watanabe S., Kotake S., Nozawa A., Muto T., Uchida S. Tumorigenicity of human BK papovavirus plaque isolates, wild-type and plaque morphology mutant, in hamsters. Int J Cancer. 1982 May 15;29(5):583–586. doi: 10.1002/ijc.2910290515. [DOI] [PubMed] [Google Scholar]
- Watanabe S., Soeda E., Uchida S., Yoshiike K. DNA rearrangement affecting expression of the BK virus transforming gene. J Virol. 1984 Jul;51(1):1–6. doi: 10.1128/jvi.51.1.1-6.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watanabe S., Yogo Y., Yoshiike K. Expression of viral early functions in rat 3Y1 cells infected with human papovavirus BK. J Virol. 1984 Jan;49(1):78–85. doi: 10.1128/jvi.49.1.78-85.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watanabe S., Yoshiike K. Change of DNA near the origin of replication enhances the transforming capacity of human papovavirus BK. J Virol. 1982 Jun;42(3):978–985. doi: 10.1128/jvi.42.3.978-985.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watanabe S., Yoshiike K., Nozawa A., Yuasa Y., Uchida S. Viable deletion mutant of human papovavirus BK that induces insulinomas in hamsters. J Virol. 1979 Dec;32(3):934–942. doi: 10.1128/jvi.32.3.934-942.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiher H., König M., Gruss P. Multiple point mutations affecting the simian virus 40 enhancer. Science. 1983 Feb 11;219(4585):626–631. doi: 10.1126/science.6297005. [DOI] [PubMed] [Google Scholar]
- Yang R. C., Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science. 1979 Oct 26;206(4417):456–462. doi: 10.1126/science.228391. [DOI] [PubMed] [Google Scholar]