Abstract
The complete sequence of the RNA which encodes the major outer-shell-neutralizing antigen (VP2) of bluetongue virus serotype 10 was determined from overlapping cDNA clones inserted into pBR322. The segment L2 RNA was 2,926 base pairs long (1.87 X 10(6) daltons) and had, in one strand, an open reading frame capable of coding for a protein that had a calculated size of 111,122 daltons (956 amino acids) and a +11.5 net charge. The coding strands of both the L2 gene and the group-specific L3 gene of bluetongue virus serotype 17 (M. Purdy, J. Petre, and P. Roy, J. Virol. 51:754-759, 1984) had common sequences of some six nucleotides at their 5' termini (namely, GUUAAA...) and eight nucleotides (namely, ...ACACUUAC) at their 3' termini. Both had short 5' noncoding regions with AUG codons at residues 20 to 22 (L2) and 18 to 20 (L3). The sequences flanking these AUG codons were similar (A/GCCAUGG). The 3' noncoding regions were longer (36 nucleotides for L2, 49 nucleotides for L3). The predicted amino acid sequence of the L2, compared with the similarly sized L3 gene product, was rich in cysteine residues and charged amino acids.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bishop D. H., Huddleston J. A., Brownlee G. G. The complete sequence of RNA segment 2 of influenza A/NT/60/68 P1 protein. Nucleic Acids Res. 1982 Feb 25;10(4):1335–1343. doi: 10.1093/nar/10.4.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huismans H., Erasmus B. J. Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort J Vet Res. 1981 Jun;48(2):51–58. [PubMed] [Google Scholar]
- Kahlon J., Sugiyama K., Roy P. Molecular basis of bluetongue virus neutralization. J Virol. 1983 Dec;48(3):627–632. doi: 10.1128/jvi.48.3.627-632.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981 Oct 24;9(20):5233–5252. doi: 10.1093/nar/9.20.5233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
- Martin S. A., Pett D. M., Zweerink H. J. Studies on the topography of reovirus and bluetongue virus capsid proteins. J Virol. 1973 Jul;12(1):194–198. doi: 10.1128/jvi.12.1.194-198.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Purdy M., Petre J., Roy P. Cloning of the bluetongue virus L3 gene. J Virol. 1984 Sep;51(3):754–759. doi: 10.1128/jvi.51.3.754-759.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rao C. D., Kiuchi A., Roy P. Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus. J Virol. 1983 May;46(2):378–383. doi: 10.1128/jvi.46.2.378-383.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugiyama K., Bishop D. H., Roy P. Analyses of the genomes of bluetongue viruses recovered in the United States. I. Oligonucleotide fingerprint studies that indicate the existence of naturally occurring reassortant BTV isolates. Virology. 1981 Oct 15;114(1):210–217. doi: 10.1016/0042-6822(81)90266-x. [DOI] [PubMed] [Google Scholar]
- Verwoerd D. W., Els H. J., De Villiers E. M., Huismans H. Structure of the bluetongue virus capsid. J Virol. 1972 Oct;10(4):783–794. doi: 10.1128/jvi.10.4.783-794.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]