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. 1983 Apr;46(1):302–306. doi: 10.1128/jvi.46.1.302-306.1983

Aberrant Distribution of Human Adenovirus Type 2 Late Proteins in Monkey Kidney Cells

Constance L Cepko 1, Phillip A Sharp 2
PMCID: PMC255123  PMID: 6402607

Abstract

Monkey kidney cells (CV-C) infected with adenovirus type 2 displayed an aberrant distribution of 100K, 100K-hexon complex, hexon monomers, hexon trimers, penton base, and fiber proteins, relative to the patterns observed in adenovirus type 2-infected human cells. Human cell patterns were observed in CV-C cells when mutants selected for growth on monkey cells were used.

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Selected References

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  1. Axelrod N. Phosphoproteins of adenovirus 2. Virology. 1978 Jun 15;87(2):366–383. doi: 10.1016/0042-6822(78)90141-1. [DOI] [PubMed] [Google Scholar]
  2. Baum S. G., Horwitz M. S., Maizel J. V., Jr Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40. J Virol. 1972 Aug;10(2):211–219. doi: 10.1128/jvi.10.2.211-219.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cepko C. L., Changelian P. S., Sharp P. A. Immunoprecipitation with two-dimensional pools as a hybridoma screening technique: production and characterization of monoclonal antibodies against adenovirus 2 proteins. Virology. 1981 Apr 30;110(2):385–401. doi: 10.1016/0042-6822(81)90069-6. [DOI] [PubMed] [Google Scholar]
  4. Cepko C. L., Sharp P. A. Assembly of adenovirus major capsid protein is mediated by a nonvirion protein. Cell. 1982 Dec;31(2 Pt 1):407–415. doi: 10.1016/0092-8674(82)90134-9. [DOI] [PubMed] [Google Scholar]
  5. Cepko C. L., Whetstone C. A., Sharp P. A. Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J Clin Microbiol. 1983 Feb;17(2):360–364. doi: 10.1128/jcm.17.2.360-364.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedman M. P., Lyons M. J., Ginsberg H. S. Biochemical consequences of type 2 adenovirus and Simian virus 40 double infections of African green monkey kidney cells. J Virol. 1970 May;5(5):586–597. doi: 10.1128/jvi.5.5.586-597.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  8. Klein H., Maltzman W., Levine A. J. Structure-function relationships of the adenovirus DNA-binding protein. J Biol Chem. 1979 Nov 10;254(21):11051–11060. [PubMed] [Google Scholar]
  9. Klessig D. F., Anderson C. W. Block to multiplication of adenovirus serotype 2 in monkey cells. J Virol. 1975 Dec;16(6):1650–1668. doi: 10.1128/jvi.16.6.1650-1668.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klessig D. F., Chow L. T. Incomplete splicing and deficient accumulation of the fiber messenger RNA in monkey cells infected by human adenovirus type 2. J Mol Biol. 1980 May 15;139(2):221–242. doi: 10.1016/0022-2836(80)90306-x. [DOI] [PubMed] [Google Scholar]
  11. Klessig D. F., Grodzicker T. Mutations that allow human Ad2 and Ad5 to express late genes in monkey cells map in the viral gene encoding the 72K DNA binding protein. Cell. 1979 Aug;17(4):957–966. doi: 10.1016/0092-8674(79)90335-0. [DOI] [PubMed] [Google Scholar]
  12. Klessig D. F., Hassell J. A. Characterization of a variant of human adenovirus type 2 which multiples efficiently in simian cells. J Virol. 1978 Dec;28(3):945–956. doi: 10.1128/jvi.28.3.945-956.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klessig D. F. Isolation of a variant of human adenovirus serotype 2 that multiplies efficiently on monkey cells. J Virol. 1977 Mar;21(3):1243–1246. doi: 10.1128/jvi.21.3.1243-1246.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lewis A. M., Jr, Levin M. J., Wiese W. H., Crumpacker C. S., Henry P. H. A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128–1135. doi: 10.1073/pnas.63.4.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lewis A. M., Jr, Levine A. S., Crumpacker C. S., Levin M. J., Samaha R. J., Henry P. H. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties. J Virol. 1973 May;11(5):655–664. doi: 10.1128/jvi.11.5.655-664.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oosterom-Dragon E. A., Ginsberg H. S. Characterization of two temperature-sensitive mutants of type 5 adenovirus with mutations in the 100,000-dalton protein gene. J Virol. 1981 Nov;40(2):491–500. doi: 10.1128/jvi.40.2.491-500.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Oosterom-Dragon E. A., Ginsberg H. S. Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein. J Virol. 1980 Mar;33(3):1203–1207. doi: 10.1128/jvi.33.3.1203-1207.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. RABSON A. S., O'CONOR G. T., BEREZESKY I. K., PAUL F. J. ENHANCEMENT OF ADENOVIRUS GROWTH IN AFRICAN GREEN MONKEY KIDNEY CELL CULTURES BY SV40. Proc Soc Exp Biol Med. 1964 May;116:187–190. doi: 10.3181/00379727-116-29197. [DOI] [PubMed] [Google Scholar]
  19. Russell W. C., Patel G., Precious B., Sharp I., Gardner P. S. Monoclonal antibodies against adenovirus type 5: preparation and preliminary characterization. J Gen Virol. 1981 Oct;56(Pt 2):393–408. doi: 10.1099/0022-1317-56-2-393. [DOI] [PubMed] [Google Scholar]
  20. Scheidtmann K. H., Kaiser A., Carbone A., Walter G. Phosphorylation of threonine in the proline-rich carboxy-terminal region of simian virus 40 large T antigen. J Virol. 1981 Apr;38(1):59–69. doi: 10.1128/jvi.38.1.59-69.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Solomon F., Magendantz M., Salzman A. Identification with cellular microtubules of one of the co-assemlbing microtubule-associated proteins. Cell. 1979 Oct;18(2):431–438. doi: 10.1016/0092-8674(79)90062-x. [DOI] [PubMed] [Google Scholar]
  22. Sugawara K., Gilead Z., Wold W. S., Green M. Immunofluorescence study of the adenovirus type 2 single-stranded DNA binding protein in infected and transformed cells. J Virol. 1977 May;22(2):527–539. doi: 10.1128/jvi.22.2.527-539.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Van Roy F., Fransen L., Fiers W. Phosphorylation patterns of tumour antigens in cells lytically infected or transformed by simian virus 40. J Virol. 1981 Oct;40(1):28–44. doi: 10.1128/jvi.40.1.28-44.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Velicer L. F., Ginsberg H. S. Synthesis, transport, and morphogenesis of type adenovirus capsid proteins. J Virol. 1970 Mar;5(3):338–352. doi: 10.1128/jvi.5.3.338-352.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Willians J. F., Young C. S., Austin P. E. Genetic analysis of human adenovirus type 5 in permissive and nonpermissive cells. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):427–437. doi: 10.1101/sqb.1974.039.01.055. [DOI] [PubMed] [Google Scholar]
  26. Zorn G. A., Anderson C. W. Adenovirus type 2 expresses fiber in monkey-human hybrids and reconstructed cells. J Virol. 1981 Feb;37(2):759–769. doi: 10.1128/jvi.37.2.759-769.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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