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. 1983 Apr;46(1):311–316. doi: 10.1128/jvi.46.1.311-316.1983

Identification of an exposed region of the immunogenic capsid polypeptide VP1 on foot-and-mouth disease virus.

B H Robertson, D M Moore, M J Grubman, D G Kleid
PMCID: PMC255125  PMID: 6186823

Abstract

Iodination of intact foot-and-mouth disease virus results in the selective labeling of VP1, substantiating its exposed location on the virion. A comparison of tryptic peptides revealed that a single tyrosine-containing peptide was labeled with iodine on intact or protease-cleaved virus. The labeled peptide from intact and protease-cleaved virus was characterized by molecular weight sizing and sequence analysis. Carboxypeptidase digestion of intact VP1, limited trypsin-cleaved VP1, and VP1 purified from bacterially contaminated tissue cultures yielded carboxyterminal residues of leucine, valine-arginine, and serine-alanine, respectively. The correlation of these findings with previous data on the amino acid sequence derived from nucleotide sequencing of serotypes A12 and O1 of foot-and-mouth disease virus VP1 places the probable exposed antigenic region of VP1 in a serotype-variable region including residues 136 through 144.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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