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. 1983 Apr;46(1):317–320. doi: 10.1128/jvi.46.1.317-320.1983

Gene Mapping of Rotavirus Double-Stranded RNA Segments by Northern Blot Hybridization: Application to Segments 7, 8, and 9

Michael L Dyall-Smith 1, Ahmed A Azad 2, Ian H Holmes 1
PMCID: PMC255126  PMID: 6298465

Abstract

Cloned DNA copies of double-stranded RNA segments 7, 8, and 9 of UK bovine rotavirus were nick-translated with [α-32P]ATP and hybridized to double-stranded RNA of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o-aminophenylthioether paper. Specific hybridization of the UK calf clones to the separated RNA segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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