Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1983 Apr;46(1):333–336. doi: 10.1128/jvi.46.1.333-336.1983

Alignment of the Genome of Monkey B-Lymphotropic Papovavirus to the Genomes of Simian Virus 40 and BK Virus

Tadahito Kanda 1, Kunito Yoshiike 1, Kenneth K Takemoto 1
PMCID: PMC255129  PMID: 6298468

Abstract

We located the origin of DNA replication of African green monkey B-lymphotropic papovavirus DNA by analyzing pulse-labeled form I DNA. With the replication origin used as a reference point, the B-lymphotropic papovavirus genome was aligned with the genomes of simian virus 40 and BK virus from DNA homology between specific fragments hybridized under low-stringency conditions. From the results of these experiments, it was possible to deduce the correlation between the physical and functional maps of the B-lymphotropic papovavirus genome.

Full text

PDF
333

Images in this article

335Image
on p.335

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brade L., Müller-Lantzsch N., zur Hausen H. B-lymphotropic papovavirus and possibility of infections in humans. J Med Virol. 1981;6(4):301–308. doi: 10.1002/jmv.1890060405. [DOI] [PubMed] [Google Scholar]
  2. Brade L., Vogl W., Gissman L., zur Hausen H. Propagation of B-lymphotropic papovavirus (LPV) in human B-lymphoma cells and characterization of its DNA. Virology. 1981 Oct 15;114(1):228–235. doi: 10.1016/0042-6822(81)90268-3. [DOI] [PubMed] [Google Scholar]
  3. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  4. Howley P. M., Israel M. A., Law M. F., Martin M. A. A rapid method for detecting and mapping homology between heterologous DNAs. Evaluation of polyomavirus genomes. J Biol Chem. 1979 Jun 10;254(11):4876–4883. [PubMed] [Google Scholar]
  5. Law M. F., Martin J. D., Takemoto K. K., Howley P. M. The colinear alignment of the genomes of papovaviruses JC, BK, and SV40. Virology. 1979 Jul 30;96(2):576–587. doi: 10.1016/0042-6822(79)90113-2. [DOI] [PubMed] [Google Scholar]
  6. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  8. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  9. Takemoto K. K., Furuno A., Kato K., Yoshiike K. Biological and biochemical studies of African green monkey lymphotropic papovavirus. J Virol. 1982 May;42(2):502–509. doi: 10.1128/jvi.42.2.502-509.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. zur Hausen H., Gissmann L. Lymphotropic papovaviruses isolated from African green monkey and human cells. Med Microbiol Immunol. 1979 Aug;167(3):137–153. doi: 10.1007/BF02121180. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES