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. 1987 Jan;61(1):214–217. doi: 10.1128/jvi.61.1.214-217.1987

Direct role of viral hemagglutinin in B-cell mitogenesis by influenza viruses.

P Poumbourios, E M Anders, A A Scalzo, D O White, A W Hampson, D C Jackson
PMCID: PMC255243  PMID: 3491221

Abstract

The mitogenic activity of influenza virus is a function of the hemagglutinin (HA) molecule. Purified HA is mitogenic for murine B lymphocytes but not T lymphocytes. Furthermore, like the intact virus, HA of the H2 (but not H3) subtype is mitogenic only for B cells expressing the class II major histocompatibility complex glycoprotein I-E. Since virus bearing uncleaved HA is as mitogenic as virus bearing cleaved HA, the membrane fusion activity of the HA molecule is not involved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anders E. M., Peppard P. M., Burns W. H., White D. O. In vitro antibody response to influenza virus. I. T cell dependence of secondary response to hemagglutinin. J Immunol. 1979 Sep;123(3):1356–1361. [PubMed] [Google Scholar]
  2. Anders E. M., Scalzo A. A., Rogers G. N., White D. O. Relationship between mitogenic activity of influenza viruses and the receptor-binding specificity of their hemagglutinin molecules. J Virol. 1986 Nov;60(2):476–482. doi: 10.1128/jvi.60.2.476-482.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anders E. M., Scalzo A. A., White D. O. Influenza viruses are T cell-independent B cell mitogens. J Virol. 1984 Jun;50(3):960–963. doi: 10.1128/jvi.50.3.960-963.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Armstrong R. B., Butchko G. M., Kiley S. C., Phelan M. A., Ennis F. A. Mitogenicity of influenza hemagglutinin glycoproteins and influenza viruses bearing H2-hemagglutinin. Infect Immun. 1981 Oct;34(1):140–143. doi: 10.1128/iai.34.1.140-143.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Butchko G. M., Armstrong R. B., Martin W. J., Ennis F. A. Influenza A viruses of the H2N2 subtype are lymphocyte mitogens. Nature. 1978 Jan 5;271(5640):66–67. doi: 10.1038/271066a0. [DOI] [PubMed] [Google Scholar]
  6. Homma M., Ouchi M. Trypsin action on the growth of Sendai virus in tissue culture cells. 3. Structural difference of Sendai viruses grown in eggs and tissue culture cells. J Virol. 1973 Dec;12(6):1457–1465. doi: 10.1128/jvi.12.6.1457-1465.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang R. T., Rott R., Klenk H. D. Influenza viruses cause hemolysis and fusion of cells. Virology. 1981 Apr 15;110(1):243–247. doi: 10.1016/0042-6822(81)90030-1. [DOI] [PubMed] [Google Scholar]
  8. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  9. Kida H., Brown L. E., Webster R. G. Biological activity of monoclonal antibodies to operationally defined antigenic regions on the hemagglutinin molecule of A/Seal/Massachusetts/1/80 (H7N7) influenza virus. Virology. 1982 Oct 15;122(1):38–47. doi: 10.1016/0042-6822(82)90375-0. [DOI] [PubMed] [Google Scholar]
  10. Kizaka S., Goodman-Snitkoff G., McSharry J. J. Sendai virus glycoproteins are T cell-dependent B cell mitogens. Infect Immun. 1983 May;40(2):592–600. doi: 10.1128/iai.40.2.592-600.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klenk H. D., Rott R., Orlich M., Blödorn J. Activation of influenza A viruses by trypsin treatment. Virology. 1975 Dec;68(2):426–439. doi: 10.1016/0042-6822(75)90284-6. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Scalzo A. A., Anders E. M. Influenza viruses as lymphocyte mitogens. I. B cell mitogenesis by influenza A viruses of the H2 and H6 subtypes is controlled by the I-E/C subregion of the major histocompatibility complex. J Immunol. 1985 Feb;134(2):757–760. [PubMed] [Google Scholar]
  14. Scalzo A. A., Anders E. M. Influenza viruses as lymphocyte mitogens. II. Role of I-E molecules in B cell mitogenesis by influenza A viruses of the H2 and H6 subtypes. J Immunol. 1985 Nov;135(5):3524–3529. [PubMed] [Google Scholar]
  15. Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]
  16. White J., Matlin K., Helenius A. Cell fusion by Semliki Forest, influenza, and vesicular stomatitis viruses. J Cell Biol. 1981 Jun;89(3):674–679. doi: 10.1083/jcb.89.3.674. [DOI] [PMC free article] [PubMed] [Google Scholar]

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