Abstract
Mus musculus subsp. musculus (Czech II) mammary tumor DNA frequently contains an integrated proviral genome of the mouse mammary tumor virus (MMTV) within a specific 0.5-kilobase-pair region of the cellular genome (designated int-3). Viral integration at this site results in activation of expression of an adjacent cellular gene. We mapped int-3 to mouse chromosome 17 by analysis of PstI-restricted cellular DNAs from mouse-hamster somatic cell hybrids. Restriction analysis of cellular DNA from (C3H/OuJ X Czech II) X Czech II backcross mice established the gene order T-H-2-int-3. These results demonstrated that the int-3 locus is distinct from two other common integration regions for mouse mammary tumor virus (designated int-1 and int-2) in mammary tumor DNA and suggest that several cellular genes may be at risk for virally induced activation during mammary tumor development.
Full text
PDF![218](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b2/255246/84be8dae5052/jvirol00092-0234.png)
![219](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b2/255246/c832cb68ea03/jvirol00092-0235.png)
![220](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03b2/255246/e93a780241a8/jvirol00092-0236.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Callahan R., Drohan W., Gallahan D., D'Hoostelaere L., Potter M. Novel class of mouse mammary tumor virus-related DNA sequences found in all species of Mus, including mice lacking the virus proviral genome. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4113–4117. doi: 10.1073/pnas.79.13.4113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen J. C., Murphey-Corb M. Targeted integration of baboon endogenous virus in the BEVI locus on human chromosome 6. Nature. 1983 Jan 13;301(5896):129–132. doi: 10.1038/301129a0. [DOI] [PubMed] [Google Scholar]
- Davis M. M., Cohen D. I., Nielsen E. A., Steinmetz M., Paul W. E., Hood L. Cell-type-specific cDNA probes and the murine I region: the localization and orientation of Ad alpha. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2194–2198. doi: 10.1073/pnas.81.7.2194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dickson C., Smith R., Brookes S., Peters G. Tumorigenesis by mouse mammary tumor virus: proviral activation of a cellular gene in the common integration region int-2. Cell. 1984 Jun;37(2):529–536. doi: 10.1016/0092-8674(84)90383-0. [DOI] [PubMed] [Google Scholar]
- Escot C., Hogg E., Callahan R. Mammary tumorigenesis in feral Mus cervicolor popaeus. J Virol. 1986 May;58(2):619–625. doi: 10.1128/jvi.58.2.619-625.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gallahan D., Callahan R. Mammary tumorigenesis in feral mice: identification of a new int locus in mouse mammary tumor virus (Czech II)-induced mammary tumors. J Virol. 1987 Jan;61(1):66–74. doi: 10.1128/jvi.61.1.66-74.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gallahan D., Escot C., Hogg E., Callahan R. Mammary tumorigenesis in feral species of the genus Mus. Curr Top Microbiol Immunol. 1986;127:354–361. doi: 10.1007/978-3-642-71304-0_43. [DOI] [PubMed] [Google Scholar]
- Kozak C. A. Genetic mapping of a mouse chromosomal locus required for mink cell focus-forming virus replication. J Virol. 1983 Oct;48(1):300–303. doi: 10.1128/jvi.48.1.300-303.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kozak C. A. Retroviruses as chromosomal genes in the mouse. Adv Cancer Res. 1985;44:295–336. doi: 10.1016/s0065-230x(08)60030-5. [DOI] [PubMed] [Google Scholar]
- Lemons R. S., Nash W. G., O'Brien S. J., Benveniste R. E., Sherr C. J. A gene (Bevi) on human chromosome 6 is an integration site for baboon type C DNA provirus in human cells. Cell. 1978 Aug;14(4):995–1005. doi: 10.1016/0092-8674(78)90353-7. [DOI] [PubMed] [Google Scholar]
- Lemons R. S., O'Brien S. J., Sherr C. J. A new genetic locus, Bevi, on human chromosome 6 which controls the replication of baboon type C virus in human cells. Cell. 1977 Sep;12(1):251–262. doi: 10.1016/0092-8674(77)90203-3. [DOI] [PubMed] [Google Scholar]
- Nusse R., Varmus H. E. Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell. 1982 Nov;31(1):99–109. doi: 10.1016/0092-8674(82)90409-3. [DOI] [PubMed] [Google Scholar]
- Nusse R., van Ooyen A., Cox D., Fung Y. K., Varmus H. Mode of proviral activation of a putative mammary oncogene (int-1) on mouse chromosome 15. Nature. 1984 Jan 12;307(5947):131–136. doi: 10.1038/307131a0. [DOI] [PubMed] [Google Scholar]
- Peters G., Brookes S., Smith R., Dickson C. Tumorigenesis by mouse mammary tumor virus: evidence for a common region for provirus integration in mammary tumors. Cell. 1983 Jun;33(2):369–377. doi: 10.1016/0092-8674(83)90418-x. [DOI] [PubMed] [Google Scholar]
- Peters G., Kozak C., Dickson C. Mouse mammary tumor virus integration regions int-1 and int-2 map on different mouse chromosomes. Mol Cell Biol. 1984 Feb;4(2):375–378. doi: 10.1128/mcb.4.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peters G., Lee A. E., Dickson C. Activation of cellular gene by mouse mammary tumour virus may occur early in mammary tumour development. Nature. 1984 May 17;309(5965):273–275. doi: 10.1038/309273a0. [DOI] [PubMed] [Google Scholar]
- Robbins J. M., Gallahan D., Hogg E., Kozak C., Callahan R. An endogenous mouse mammary tumor virus genome common in inbred mouse strains is located on chromosome 6. J Virol. 1986 Feb;57(2):709–713. doi: 10.1128/jvi.57.2.709-713.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Röhme D., Fox H., Herrmann B., Frischauf A. M., Edström J. E., Mains P., Silver L. M., Lehrach H. Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes. Cell. 1984 Mar;36(3):783–788. doi: 10.1016/0092-8674(84)90358-1. [DOI] [PubMed] [Google Scholar]
- Silver L. M. Genetic organization of the mouse t complex. Cell. 1981 Dec;27(2 Pt 1):239–240. doi: 10.1016/0092-8674(81)90406-2. [DOI] [PubMed] [Google Scholar]