Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1983 Aug;47(2):370–375. doi: 10.1128/jvi.47.2.370-375.1983

Ionic and nonionic interactions in adenoviral nucleoprotein complexes.

M J Fedor, E Daniell
PMCID: PMC255270  PMID: 6620457

Abstract

Ionic and nonionic interactions between the adenoviral histone-like proteins and DNA were examined by determining effects of ionic strength and urea concentration on disruption of viral nucleoprotein. The viral proteins were as susceptible to dissociation by salt in the presence of urea as histones. Nonionic interactions between viral proteins appeared more extensive than those between histones.

Full text

PDF
370

Images in this article

373Image
on p.373
374Image
on p.374

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amin M., Mirza A., Weber J. Genetic analysis of adenovirus type 2. VII. Cleavage-modified affinity for DNA of internal virion proteins. Virology. 1977 Jul 1;80(1):83–97. doi: 10.1016/0042-6822(77)90382-8. [DOI] [PubMed] [Google Scholar]
  2. Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartley J. A., Chalkley R. The binding of deoxyribonucleic acid and histone in native nucleohistone. J Biol Chem. 1972 Jun 10;247(11):3647–3655. [PubMed] [Google Scholar]
  4. Black B. C., Center M. S. DNA-binding properties of the major core protein of adenovirus 2. Nucleic Acids Res. 1979;6(6):2339–2353. doi: 10.1093/nar/6.6.2339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carlson R. D., Olins A. L., Olins D. E. Urea denaturation of chromatin periodic structure. Biochemistry. 1975 Jul 15;14(14):3122–3125. doi: 10.1021/bi00685a013. [DOI] [PubMed] [Google Scholar]
  6. Daniell E., Fedor M. J. Transcription of adenovirus cores in vitro: major RNA products differ from those made from a DNA template. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1834–1838. doi: 10.1073/pnas.79.6.1834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Daniell E., Groff D. E., Fedor M. J. Adenovirus chromatin structure at different stages of infection. Mol Cell Biol. 1981 Dec;1(12):1094–1105. doi: 10.1128/mcb.1.12.1094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eickbush T. H., Moudrianakis E. N. The histone core complex: an octamer assembled by two sets of protein-protein interactions. Biochemistry. 1978 Nov 14;17(23):4955–4964. doi: 10.1021/bi00616a016. [DOI] [PubMed] [Google Scholar]
  9. Everitt E., Meador S. A., Levine A. S. Synthesis and processing of the precursor to the major core protein of adenovirus type 2. J Virol. 1977 Jan;21(1):199–214. doi: 10.1128/jvi.21.1.199-214.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Everitt E., Sundquist B., Pettersson U., Philipson L. Structural proteins of adenoviruses. X. Isolation and topography of low molecular weight antigens from the virion of adenovirus type 2. Virology. 1973 Mar;52(1):130–147. doi: 10.1016/0042-6822(73)90404-2. [DOI] [PubMed] [Google Scholar]
  11. Fedor M. J., Daniell E. Acetylation of histone-like proteins of adenovirus type 5. J Virol. 1980 Sep;35(3):637–643. doi: 10.1128/jvi.35.3.637-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goodwin G. H., Sanders C., Johns E. W. A new group of chromatin-associated proteins with a high content of acidic and basic amino acids. Eur J Biochem. 1973 Sep 21;38(1):14–19. doi: 10.1111/j.1432-1033.1973.tb03026.x. [DOI] [PubMed] [Google Scholar]
  13. Harpst J. A., Ennever J. F., Russell W. C. Physical properties of nucleoprotein cores from adenovirus type 5. Nucleic Acids Res. 1977 Feb;4(2):477–490. doi: 10.1093/nar/4.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hodge L. D., Scharff M. D. Effect of adenovirus on host cell DNA synthesis in synchronized cells. Virology. 1969 Apr;37(4):554–564. doi: 10.1016/0042-6822(69)90273-6. [DOI] [PubMed] [Google Scholar]
  15. Kit G., Daniell E. Adenovirus core protein synthesis in the absence of viral DNA synthesis late in infection. J Virol. 1978 Jul;27(1):74–80. doi: 10.1128/jvi.27.1.74-80.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laver W. G. Isolation of an arginine-rich protein from particles of adenovirus type 2. Virology. 1970 Jul;41(3):488–500. doi: 10.1016/0042-6822(70)90170-4. [DOI] [PubMed] [Google Scholar]
  17. Lischwe M. A., Sung M. T. A histone-like protein from adenovirus chromatin. Nature. 1977 Jun 9;267(5611):552–554. doi: 10.1038/267552a0. [DOI] [PubMed] [Google Scholar]
  18. Lonberg-Holm K., Philipson L. Early events of virus-cell interaction in an adenovirus system. J Virol. 1969 Oct;4(4):323–338. doi: 10.1128/jvi.4.4.323-338.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mirza M. A., Weber J. Uncoating of adenovirus type 2. J Virol. 1979 May;30(2):462–471. doi: 10.1128/jvi.30.2.462-471.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ohlenbusch H. H., Olivera B. M., Tuan D., Davidson N. Selective dissociation of histones from calf thymus nucleoprotein. J Mol Biol. 1967 Apr 28;25(2):299–315. doi: 10.1016/0022-2836(67)90143-x. [DOI] [PubMed] [Google Scholar]
  21. Olins D. E., Bryan P. N., Harrington R. E., Hill W. E., Olins A. L. Conformational states of chromatin nu bodies induced by urea. Nucleic Acids Res. 1977 Jun;4(6):1911–1931. doi: 10.1093/nar/4.6.1911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Palter K. B., Alberts B. M. The use of DNA-cellulose for analyzing histone-DNA interactions. Discovery of nucleosome-like histone binding to single-stranded DNA. J Biol Chem. 1979 Nov 10;254(21):11160–11169. [PubMed] [Google Scholar]
  23. Prage L., Pettersson U., Höglund S., Lonberg-Holm K., Philipson L. Structural proteins of adenoviruses. IV. Sequential degradation of the adenovirus type 2 virion. Virology. 1970 Oct;42(2):341–358. doi: 10.1016/0042-6822(70)90278-3. [DOI] [PubMed] [Google Scholar]
  24. Russell W. C., McIntosh K., Skehel J. J. The preparation and properties of adenovirus cores. J Gen Virol. 1971 Apr;11(1):35–46. doi: 10.1099/0022-1317-11-1-35. [DOI] [PubMed] [Google Scholar]
  25. Seale R. L. In vivo assembly of newly synthesized histones. Biochemistry. 1981 Oct 27;20(22):6432–6437. doi: 10.1021/bi00525a023. [DOI] [PubMed] [Google Scholar]
  26. Sergeant A., Tigges M. A., Raskas H. J. Nucleosome-like structural subunits of intranuclear parental adenovirus type 2 DNA. J Virol. 1979 Mar;29(3):888–898. doi: 10.1128/jvi.29.3.888-898.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sung M. T., Lischwe M. A., Richards J. C., Hosokawa K. Adenovirus chromatin I. Isolation and characterization of the major core protein VII and precursor Pro-VII. J Biol Chem. 1977 Jul 25;252(14):4981–4987. [PubMed] [Google Scholar]
  28. Tate V. E., Philipson L. Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells. Nucleic Acids Res. 1979 Jun 25;6(8):2769–2785. doi: 10.1093/nar/6.8.2769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Watson D. K., Moudrianakis E. N. Histone-dependent reconstitution and nucleosomal localization of a nonhistone chromosomal protein: the H2A-specific protease. Biochemistry. 1982 Jan 19;21(2):248–256. doi: 10.1021/bi00531a008. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES