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Effects of polyamines on IgM production by HB4C5 cells. HB4C5 cells were inoculated at 1 × 105 cells/mL in ITES-ERDF medium supplemented with putrescine, spermidine, thermine, and TETA at various concentrations. The amount of IgM in culture medium was measured by ELISA. These results are expressed as the mean ± SD of four independent measurements
