Fig. 3.
Effect of thermine on proliferation and IgM productivity of HB4C5 cells. HB4C5 cells were inoculated at 1 × 105 cells/mL in ITES-ERDF medium supplemented with or without 2.0 mM thermine and cultured for 5 days. Section (a) shows the growth curves of HB4C5 cells in thermine-supplemented ITES-ERDF medium (○) and that in ITES-ERDF medium (●). Section (b) indicates IgM productivity. The white and black bars express IgM productivity of HB4C5 cells in thermine-supplemented medium and control medium, respectively. Results are expressed as the mean ± SD of two independent measurements