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. 1983 Nov;48(2):534–541. doi: 10.1128/jvi.48.2.534-541.1983

Major polypeptide of duck hepatitis B surface antigen particles.

P L Marion, S S Knight, M A Feitelson, L S Oshiro, W S Robinson
PMCID: PMC255377  PMID: 6620467

Abstract

The 40- to 50-nm pleomorphic particles found in the sera of domestic Pekin ducks infected with duck hepatitis B virus were purified by rate zonal and isopycnic centrifugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic polypeptide analysis of these particles, called duck hepatitis B surface antigen particles, revealed the major component to be a single 17,500-dalton polypeptide. This result is in contrast to polypeptide analyses of the surface antigens of related mammalian viruses, including hepatitis B, in which a major doublet of polypeptides is seen with molecular weights ranging from 23,000 to 29,000. Tryptic maps of 17,500-dalton polypeptide resembled that of the major non-glycosylated polypeptide of the adw subtype of hepatitis B surface antigen. A serological assay for antibody to the purified duck virus particles is also described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Couroucé-Pauty A. M., Soulier J. P. Further data on HBs antigen subtypes - geographical distribution. Vox Sang. 1974;27(6):533–549. doi: 10.1111/j.1423-0410.1974.tb02451.x. [DOI] [PubMed] [Google Scholar]
  2. Feitelson M. A., Marion P. L., Robinson W. S. Antigenic and structural relationships of the surface antigens of hepatitis B virus, ground squirrel hepatitis virus, and woodchuck hepatitis virus. J Virol. 1981 Aug;39(2):447–454. doi: 10.1128/jvi.39.2.447-454.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ganem D., Weiser B., Barchuk A., Brown R. J., Varmus H. E. Biological characterization of acute infection with ground squirrel hepatitis virus. J Virol. 1982 Oct;44(1):366–373. doi: 10.1128/jvi.44.1.366-373.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gerlich W. H., Feitelson M. A., Marion P. L., Robinson W. S. Structural relationships between the surface antigens of ground squirrel hepatitis virus and human hepatitis B virus. J Virol. 1980 Dec;36(3):787–795. doi: 10.1128/jvi.36.3.787-795.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerlich W. H., Robinson W. S. Hepatitis B virus contains protein attached to the 5' terminus of its complete DNA strand. Cell. 1980 Oct;21(3):801–809. doi: 10.1016/0092-8674(80)90443-2. [DOI] [PubMed] [Google Scholar]
  6. Marion P. L., Oshiro L. S., Regnery D. C., Scullard G. H., Robinson W. S. A virus in Beechey ground squirrels that is related to hepatitis B virus of humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2941–2945. doi: 10.1073/pnas.77.5.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marion P. L., Salazar F. H., Alexander J. J., Robinson W. S. Polypeptides of hepatitis B virus surface antigen produced by a hepatoma cell line. J Virol. 1979 Dec;32(3):796–802. doi: 10.1128/jvi.32.3.796-802.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mason W. S., Seal G., Summers J. Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. J Virol. 1980 Dec;36(3):829–836. doi: 10.1128/jvi.36.3.829-836.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Muller J. Y. Protocol of analysis of the components present in mono- and polyspecific antisera. Bibl Haematol. 1976;42:137–158. doi: 10.1159/000399001. [DOI] [PubMed] [Google Scholar]
  10. Oi V. T., Herzenberg L. A. Localization of murine Ig-1b and Ig-1a (IgG 2a) allotypic determinants detected with monoclonal antibodies. Mol Immunol. 1979 Dec;16(12):1005–1017. doi: 10.1016/0161-5890(79)90034-8. [DOI] [PubMed] [Google Scholar]
  11. Peterson D. L. Isolation and characterization of the major protein and glycoprotein of hepatitis B surface antigen. J Biol Chem. 1981 Jul 10;256(13):6975–6983. [PubMed] [Google Scholar]
  12. Peterson D. L., Roberts I. M., Vyas G. N. Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1530–1534. doi: 10.1073/pnas.74.4.1530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shimoda T., Shikata T., Karasawa T., Tsukagoshi S., Yoshimura M., Sakurai I. Light microscopic localization of hepatitis B virus antigens in the human pancreas. Possibility of multiplication of hepatitis B virus in the human pancreas. Gastroenterology. 1981 Dec;81(6):998–1005. [PubMed] [Google Scholar]
  14. Summers J., Smolec J. M., Snyder R. A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4533–4537. doi: 10.1073/pnas.75.9.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Summers J. Three recently described animal virus models for human hepatitis B virus. Hepatology. 1981 Mar-Apr;1(2):179–183. doi: 10.1002/hep.1840010215. [DOI] [PubMed] [Google Scholar]

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