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. 1983 Dec;48(3):616–626. doi: 10.1128/jvi.48.3.616-626.1983

An Escherichia coli gene required for bacteriophage P2-lambda interference.

D Ghisotti, S Zangrossi, G Sironi
PMCID: PMC255392  PMID: 6355505

Abstract

The gene old of bacteriophage P2 is known to (i) cause interference with phage lambda growth; (ii) kill recB- mutants of Escherichia coli after P2 infection; and (iii) determine increased sensitivity of P2 lysogenic cells to X-ray irradiation. In all of these phenomena, inhibition of protein synthesis occurs. We have isolated bacterial mutants, named pin (P2 interference), able to suppress all of the above-mentioned phenomena caused by the old+ gene product and the concurrent protein synthesis inhibition. Pin mutations are recessive, map at 12 min on the E. coli map, and identify a new gene. Satellite bacteriophage P4 does not plate on pin-3 mutant strains and causes cell lethality and protein synthesis inhibition in such mutants. P4 mutants able to grow on pin-3 strains have been isolated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barbour S. D., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes. Proc Natl Acad Sci U S A. 1970 Apr;65(4):955–961. doi: 10.1073/pnas.65.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbour S. D., Nagaishi H., Templin A., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations. Proc Natl Acad Sci U S A. 1970 Sep;67(1):128–135. doi: 10.1073/pnas.67.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barrett K. J., Gibbs W., Calendar R. A transcribing activity induced by satellite phage P4. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2986–2990. doi: 10.1073/pnas.69.10.2986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barrett K. J., Marsh M. L., Calendar R. Interactions between a satellite bacteriophage and its helper. J Mol Biol. 1976 Sep 25;106(3):683–707. doi: 10.1016/0022-2836(76)90259-x. [DOI] [PubMed] [Google Scholar]
  6. Berg C. M., Curtiss R., 3rd Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967 Jul;56(3):503–525. doi: 10.1093/genetics/56.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bertani G., Choe B. K., Lindahl G. Calcium sensitive and other mutants of bacteriophage P2. J Gen Virol. 1969 Jul;5(1):97–104. doi: 10.1099/0022-1317-5-1-97. [DOI] [PubMed] [Google Scholar]
  8. Bregegere F. Bacteriophage P2-lambda interference: inhibition of protein synthesis involves transfer RNA inactivation. J Mol Biol. 1974 Dec 15;90(3):459–467. doi: 10.1016/0022-2836(74)90228-9. [DOI] [PubMed] [Google Scholar]
  9. Brégégère F. Bacteriophage P2-lambda interference. II. Effects on the host under the control of lambda genes O and P. J Mol Biol. 1976 Jun 25;104(2):411–420. doi: 10.1016/0022-2836(76)90279-5. [DOI] [PubMed] [Google Scholar]
  10. Brégégère F. Bacteriophage P2-lambda interference. III. Essential role of an early step in the initiation of lambda replication. J Mol Biol. 1978 Jun 25;122(2):113–125. doi: 10.1016/0022-2836(78)90029-3. [DOI] [PubMed] [Google Scholar]
  11. COHEN D. A variant of phage P2 originating in Escherichia coli, strain B. Virology. 1959 Jan;7(1):112–126. doi: 10.1016/0042-6822(59)90180-1. [DOI] [PubMed] [Google Scholar]
  12. Calendar R., Lindqvist B., Sironi G., Clark A. J. Characterization of REP- mutants and their interaction with P2 phage. Virology. 1970 Jan;40(1):72–83. doi: 10.1016/0042-6822(70)90380-6. [DOI] [PubMed] [Google Scholar]
  13. Calendar R., Ljungquist E., Deho G., Usher D. C., Goldstein R., Youderian P., Sironi G., Six E. W. Lysogenization by satellite phage P4. Virology. 1981 Aug;113(1):20–38. doi: 10.1016/0042-6822(81)90133-1. [DOI] [PubMed] [Google Scholar]
  14. Dehò G. Circular genetic map of satellite bacteriophage P4. Virology. 1983 Apr 15;126(1):267–278. doi: 10.1016/0042-6822(83)90478-6. [DOI] [PubMed] [Google Scholar]
  15. Emmerson P. T. Recombination deficient mutants of Escherichia coli K12 that map between thy A and argA. Genetics. 1968 Sep;60(1):19–30. doi: 10.1093/genetics/60.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Filip C. C., Allen J. S., Gustafson R. A., Allen R. G., Walker J. R. Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli. J Bacteriol. 1974 Aug;119(2):443–449. doi: 10.1128/jb.119.2.443-449.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ghisotti D., Zangrossi S., Sironi G. X-ray sensitivity of Escherichia coli lysogenic for bacteriophage P2. Mol Gen Genet. 1979 Feb 1;169(3):229–235. doi: 10.1007/BF00382268. [DOI] [PubMed] [Google Scholar]
  18. Gibbs W., Goldstein R. N., Wiener R., Lindqvist B., Calendar R. Satellite bacteriophage P4: characterization of mutants in two essential genes. Virology. 1973 May;53(1):24–39. doi: 10.1016/0042-6822(73)90462-5. [DOI] [PubMed] [Google Scholar]
  19. Herbert A. A., Guest J. R. Biochemical and genetic studies with lysine+methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J Gen Microbiol. 1968 Oct;53(3):363–381. doi: 10.1099/00221287-53-3-363. [DOI] [PubMed] [Google Scholar]
  20. KELLY B. Localization of P2 prophage in two strains of Escherichia coli. Virology. 1963 Jan;19:32–39. doi: 10.1016/0042-6822(63)90021-7. [DOI] [PubMed] [Google Scholar]
  21. Kahn M., Hopkins A. Restriction endonuclease cleavage map of bacteriophage P4 DNA. Virology. 1978 Apr;85(2):359–363. doi: 10.1016/0042-6822(78)90444-0. [DOI] [PubMed] [Google Scholar]
  22. Kushner S. R., Nagaishi H., Clark A. J. Isolation of exonuclease VIII: the enzyme associated with sbcA indirect suppressor. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3593–3597. doi: 10.1073/pnas.71.9.3593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lam S. T., Stahl M. M., McMilin K. D., Stahl F. W. Rec-mediated recombinational hot spot activity in bacteriophage lambda. II. A mutation which causes hot spot activity. Genetics. 1974 Jul;77(3):425–433. doi: 10.1093/genetics/77.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lindahl G., Sironi G., Bialy H., Calendar R. Bacteriophage lambda; abortive infection of bacteria lysogenic for phage P2. Proc Natl Acad Sci U S A. 1970 Jul;66(3):587–594. doi: 10.1073/pnas.66.3.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Lindqvist B. H., Six E. W. Replication of bacteriophage P4 DNA in a nonlysogenic host. Virology. 1971 Jan;43(1):1–7. doi: 10.1016/0042-6822(71)90218-2. [DOI] [PubMed] [Google Scholar]
  26. Lloyd R. G., Barbour S. D. The genetic location of the sbcA gene of Escherichia coli. Mol Gen Genet. 1974;134(2):157–171. doi: 10.1007/BF00268417. [DOI] [PubMed] [Google Scholar]
  27. Malone R. E., Chattoraj D. K. The role of Chi mutations in the Spi- phenotype of phage lambda: lack of evidence for a gene delta. Mol Gen Genet. 1975 Dec 30;143(1):35–41. doi: 10.1007/BF00269418. [DOI] [PubMed] [Google Scholar]
  28. Manly K. F., Signer E. R., Radding C. M. Nonessential functions of bacteriophage lambda. Virology. 1969 Feb;37(2):177–188. doi: 10.1016/0042-6822(69)90197-4. [DOI] [PubMed] [Google Scholar]
  29. OKADA T., YANAGISAWA K., RYAN F. J. A method for securing thymineless mutants from strains of E. coli. Z Vererbungsl. 1961;92:403–412. doi: 10.1007/BF00890061. [DOI] [PubMed] [Google Scholar]
  30. SUSSMAN R., JACOB F. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage]. C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517–1519. [PubMed] [Google Scholar]
  31. Sakaki Y., Karu A. E., Linn S., Echols H. Purification and properties of the gamma-protein specified by bacteriophage lambda: an inhibitor of the host RecBC recombination enzyme. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2215–2219. doi: 10.1073/pnas.70.8.2215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sasaki I., Bertani G. Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J Gen Microbiol. 1965 Sep;40(3):365–376. doi: 10.1099/00221287-40-3-365. [DOI] [PubMed] [Google Scholar]
  33. Shinomiya S., Sakaki Y. Prophage P2 does not kill recB bacteria. Biochem Biophys Res Commun. 1979 Jan 15;86(1):167–172. doi: 10.1016/0006-291x(79)90396-6. [DOI] [PubMed] [Google Scholar]
  34. Shore D., Dehò G., Tsipis J., Goldstein R. Determination of capsid size by satellite bacteriophage P4. Proc Natl Acad Sci U S A. 1978 Jan;75(1):400–404. doi: 10.1073/pnas.75.1.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Sironi G., Bialy H., Lozeron H. A., Calendar R. Bacteriophage P2: interaction with phage lambda and with recombination-deficient bacteria. Virology. 1971 Nov;46(2):387–396. doi: 10.1016/0042-6822(71)90040-7. [DOI] [PubMed] [Google Scholar]
  36. Sironi G. Mutants of Escherichia coli unable to be lysogenized by the temperate bacteriophage P2. Virology. 1969 Feb;37(2):163–176. doi: 10.1016/0042-6822(69)90196-2. [DOI] [PubMed] [Google Scholar]
  37. Six E. W. The helper dependence of satellite bacteriophage P4: which gene functions of bacteriophage P2 are needed by P4? Virology. 1975 Sep;67(1):249–263. doi: 10.1016/0042-6822(75)90422-5. [DOI] [PubMed] [Google Scholar]
  38. Six E. PROPHAGE SUBSTITUTION AND CURING IN LYSOGENIC CELLS SUPERINFECTED WITH HETERO-IMMUNE PHAGE. J Bacteriol. 1960 Nov;80(5):728–729. doi: 10.1128/jb.80.5.728-729.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Souza L., Geisselsoder J., Hopkins A., Calender R. Physical mapping of the satellite phage P4 genome. Virology. 1978 Apr;85(2):335–342. doi: 10.1016/0042-6822(78)90442-7. [DOI] [PubMed] [Google Scholar]
  40. Sunshine M. G., Thorn M., Gibbs W., Calendar R., Kelly B. P2 phage amber mutants: characterization by use of a polarity suppressor. Virology. 1971 Dec;46(3):691–702. doi: 10.1016/0042-6822(71)90071-7. [DOI] [PubMed] [Google Scholar]
  41. Szybalski E. H., Szybalski W. Physical mapping of the att-N region of coliphage lambda: apparent oversaturation of coding capacity in the gam-ral segment. Biochimie. 1974;56(11-12):1497–1503. doi: 10.1016/s0300-9084(75)80272-0. [DOI] [PubMed] [Google Scholar]
  42. THOMAS R., BERTANI L. E. ON THE CONTROL OF THE REPLICATION OF TEMPERATE BACTERIOPHAGES SUPERINFECTING IMMUNE HOSTS. Virology. 1964 Nov;24:241–253. doi: 10.1016/0042-6822(64)90163-1. [DOI] [PubMed] [Google Scholar]
  43. Unger R. C., Clark A. J. Interaction of the recombination pathways of bacteriophage lambda and its host Escherichia coli K12: effects on exonuclease V activity. J Mol Biol. 1972 Oct 14;70(3):539–548. doi: 10.1016/0022-2836(72)90558-x. [DOI] [PubMed] [Google Scholar]
  44. Wiman M., Bertani G., Kelly B., Sasaki I. Genetic map of Escherichia coli strain C. Mol Gen Genet. 1970;107(1):1–31. doi: 10.1007/BF00433220. [DOI] [PubMed] [Google Scholar]
  45. Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]

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