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. 1984 Jan;49(1):9–13. doi: 10.1128/jvi.49.1.9-13.1984

Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.

B A Lewton, A L DeLucia, P Tegtmeyer
PMCID: PMC255418  PMID: 6317897

Abstract

Previous studies with wild-type simian virus 40 DNA have shown that the sequence 5'-GAGGC-3' directs the binding of A protein (T antigen). The functional origin of replication contains four recognition pentanucleotides each of which is separated by a single base pair and arranged a two pairs of direct repetitions that are inverted relative to each other. Analysis of A protein binding to a series of nonviable mutants progressively deleting these contact sites leads to the following conclusions: (i) stable binding of subunits of A protein to three origin pentanucleotides is not sufficient for the initiation of DNA replication, (ii) the stability of DNA binding depends on interactions between bound protein subunits, and (iii) a single pentanucleotide is sufficient to bind and orient a subunit of A protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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