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. 1984 Jan;49(1):125–131. doi: 10.1128/jvi.49.1.125-131.1984

Transcriptional mapping of the DNA polymerase gene of vaccinia virus.

P Traktman, P Sridhar, R C Condit, B E Roberts
PMCID: PMC255433  PMID: 6317886

Abstract

Vaccinia virus DNA polymerase, a single-subunit enzyme of 110,000 molecular weight, is induced early after infection. Genetic analysis suggests that the gene encoding the enzyme maps within a 15-kilobase HindIII fragment located 45 kilobases from the left-hand end of the genome. We identified the in vitro translation product with these properties and mapped the transcript by hybrid selection, RNA filter hybridization, and S1 nuclease mapping. Two mRNAs from this region, 3.4 and 3.9 kilobases in size, could be translated in vitro to yield a 110K polypeptide. The two RNAs shared a common 5' terminus and had staggered 3' ends. Sequences mapping entirely within this gene were shown to be biologically active in rescuing mutants with temperature-sensitive or drug-resistant polymerase activity to the wild-type phenotype.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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