Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1984 Jan;49(1):262–264. doi: 10.1128/jvi.49.1.262-264.1984

Reversal of simian virus 40 small-t-antigen-induced theophylline resistance.

K Rundell, M Calenoff, C Renz
PMCID: PMC255450  PMID: 6690714

Abstract

Simian virus 40 small-t antigen allows the growth of CV-1 cells in concentrations of theophylline that normally prevent cell cycling. This effect is completely overcome by two ionophores that uncouple mitochondria and by the anti-mitochondrial drug methylglyoxal bis-(guanylhydrazone).

Full text

PDF
262

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball E. H., Singer S. J. Mitochondria are associated with microtubules and not with intermediate filaments in cultured fibroblasts. Proc Natl Acad Sci U S A. 1982 Jan;79(1):123–126. doi: 10.1073/pnas.79.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bouck N., Beales N., Shenk T., Berg P., di Mayorca G. New region of the simian virus 40 genome required for efficient viral transformation. Proc Natl Acad Sci U S A. 1978 May;75(5):2473–2477. doi: 10.1073/pnas.75.5.2473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burckhardt G., Pietrzyk C. The influence of an uncoupler on amino acid accumulation in Ehrlich mouse ascites tumor cells. Biochim Biophys Acta. 1980 Sep 18;601(2):403–414. doi: 10.1016/0005-2736(80)90544-1. [DOI] [PubMed] [Google Scholar]
  4. Byczkowski J. Z., Salamon W., Harlos J. P., Porter C. W. Actions of bis(guanylhydrazones) on isolated rat liver mitochondria. Biochem Pharmacol. 1981 Oct;30(20):2851–2860. doi: 10.1016/0006-2952(81)90425-1. [DOI] [PubMed] [Google Scholar]
  5. Fillingame R. H., Morris D. R. S-adenosyl-L-methionine decarboxylase during lymphocyte transformation: decreased degradation in the presence of a specific inhibitor. Biochem Biophys Res Commun. 1973 Jun 8;52(3):1020–1025. doi: 10.1016/0006-291x(73)91039-5. [DOI] [PubMed] [Google Scholar]
  6. Graessmann A., Graessmann M., Tjian R., Topp W. C. Simian virus 40 small-t protein is required for loss of actin cable networks in rat cells. J Virol. 1980 Mar;33(3):1182–1191. doi: 10.1128/jvi.33.3.1182-1191.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  8. Lever J. E. Membrane potential and neutral amino acid transport in plasma membrane vesicles from Simian virus 40 transformed mouse fibroblasts. Biochemistry. 1977 Sep 20;16(19):4328–4334. doi: 10.1021/bi00638a031. [DOI] [PubMed] [Google Scholar]
  9. Martin R. G., Setlow V. P., Edwards C. A., Vembu D. The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells. Cell. 1979 Jul;17(3):635–643. doi: 10.1016/0092-8674(79)90271-x. [DOI] [PubMed] [Google Scholar]
  10. Mose-Larsen P., Bravo R., Fey S. J., Small J. V., Celis J. E. Putative association of mitochondria with a subpopulation of intermediate-sized filaments in cultured human skin fibroblasts. Cell. 1982 Dec;31(3 Pt 2):681–692. doi: 10.1016/0092-8674(82)90323-3. [DOI] [PubMed] [Google Scholar]
  11. Pine M. J., DiPaolo J. A. The antimitochondrial action of 2-choloro-4', 4"-bis(2-imidazolin-2-yl)terephthalanilide and methylglyoxal bis(guanylhydrazone). Cancer Res. 1966 Jan;26(1):18–25. [PubMed] [Google Scholar]
  12. Rundell K., Cox J. Simian virus 40 t antigen affects the sensitivity of cellular DNA synthesis to theophylline. J Virol. 1979 Apr;30(1):394–396. doi: 10.1128/jvi.30.1.394-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sleigh M. J., Topp W. C., Hanich R., Sambrook J. F. Mutants of SV40 with an altered small t protein are reduced in their ability to transform cells. Cell. 1978 May;14(1):79–88. doi: 10.1016/0092-8674(78)90303-3. [DOI] [PubMed] [Google Scholar]
  14. Smith J. B., Rozengurt E. Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5560–5564. doi: 10.1073/pnas.75.11.5560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Soprano K. J., Galanti N., Jonak G. J., McKercher S., Pipas J. M., Peden K. W., Baserga R. Mutational analysis of simian virus 40 T antigen: stimulation of cellular DNA synthesis and activation of rRNA genes by mutants with deletions in the T-antigen gene. Mol Cell Biol. 1983 Feb;3(2):214–219. doi: 10.1128/mcb.3.2.214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang Y. C., Hearing P., Rundell K. Cellular proteins associated with simian virus 40 early gene products in newly infected cells. J Virol. 1979 Oct;32(1):147–154. doi: 10.1128/jvi.32.1.147-154.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES