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. 1984 Jan;49(1):279–282. doi: 10.1128/jvi.49.1.279-282.1984

Isolation and partial chemical characterization of a 64,000-dalton glycoprotein of human cytomegalovirus.

B R Clark, J A Zaia, L Balce-Directo, Y P Ting
PMCID: PMC255455  PMID: 6317893

Abstract

A guanidinium chloride extract of [3H]glucosamine- and [35S]methionine-labeled virions plus dense bodies of human cytomegalovirus (Towne) was separated by reverse-phase high-pressure liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate revealed the major peak to be a glycoprotein with a relative mass of 64,000. This glycoprotein (HCMVgp64) was characterized by amino acid analysis and a high-pressure liquid chromatographic map of its tryptic peptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barzilai R., Lazarus L. H., Goldblum N. Viscosity-density gradient for purification of foot-and-mouth disease virus. Arch Gesamte Virusforsch. 1972;36(1):141–146. doi: 10.1007/BF01250304. [DOI] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Del Valle U., Shively J. E. Two-column system for determination of glucosamine, galactosamine, and amino acids on a Beckman 121MB amino acid analyzer: separation of the anomers of glucosamine and galactosamine. Anal Biochem. 1979 Jul 1;96(1):77–83. doi: 10.1016/0003-2697(79)90556-6. [DOI] [PubMed] [Google Scholar]
  4. Fiala M., Honess R. W., Heiner D. C., Heine J. W., Jr, Murnane J., Wallace R., Guze L. B. Cytomegalovirus proteins. I. Polypeptides of virions and dense bodies. J Virol. 1976 Jul;19(1):243–254. doi: 10.1128/jvi.19.1.243-254.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kim K. S., Sapienza V. J., Carp R. I., Moon H. M. Analysis of structural polypeptides of purified human cytomegalovirus. J Virol. 1976 Dec;20(3):604–611. doi: 10.1128/jvi.20.3.604-611.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pereira L., Hoffman M., Cremer N. Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera. Infect Immun. 1982 Jun;36(3):933–942. doi: 10.1128/iai.36.3.933-942.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pereira L., Hoffman M., Gallo D., Cremer N. Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants. Infect Immun. 1982 Jun;36(3):924–932. doi: 10.1128/iai.36.3.924-932.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sarov I., Abady I. The morphogenesis of human cytomegalovirus. Isolation and polypeptide characterization of cytomegalovirions and dense bodies. Virology. 1975 Aug;66(2):464–473. doi: 10.1016/0042-6822(75)90218-4. [DOI] [PubMed] [Google Scholar]
  9. Stinski M. F. Human cytomegalovirus: glycoproteins associated with virions and dense bodies. J Virol. 1976 Aug;19(2):594–609. doi: 10.1128/jvi.19.2.594-609.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Zaia J. A., Oxman M. N. Antibody to varicella-zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde-fixed target cells. J Infect Dis. 1977 Oct;136(4):519–530. doi: 10.1093/infdis/136.4.519. [DOI] [PubMed] [Google Scholar]

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