Abstract
A previous report described the infection of the chick embryo with Neisseria meningitidis. Although death of embryos was preceded by multiplication of the organisms, the possibility exists that death is caused by the endotoxin contained in the inoculum or released by the bacteria lysing in vivo. A second important condition for the establishment of a satisfactory sero-protection test is that the embryo should act as a host and not merely as a growth medium for N. meningitidis.
Results of tests with cortisone-treated embryos show that the pathogenic effect of live meningococcal challenge is mainly due to infection elicited by the meningococci and not to toxaemia. Also, the rate of clearance of meningococci from the blood of the embryo, increased by the addition of meningococcal immune serum, suggests that at this stage of embryonic development (12 days) the phagocytes are active in combating meningococcal infection.
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Selected References
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- Buddingh G. J., Polk A. D. EXPERIMENTAL MENINGOCOCCUS INFECTION OF THE CHICK EMBRYO. J Exp Med. 1939 Oct 31;70(5):485–498. doi: 10.1084/jem.70.5.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FINKELSTEIN R. A. OBSERVATIONS ON MODE OF ACTION OF ENDOTOXIN IN CHICK EMBRYOS. Proc Soc Exp Biol Med. 1964 Mar;115:702–707. doi: 10.3181/00379727-115-29012. [DOI] [PubMed] [Google Scholar]
- HOOK E. W., LUTTRELL C. N., WAGNER R. R. Toxicity of bacterial endotoxins for the chicken embryo: pathological and biological studies. Bull Johns Hopkins Hosp. 1961 Jan;108:21–35. [PubMed] [Google Scholar]
- Kenny C. P., Ashton F. E., Diena B. B., Greenberg L. A chemically defined protein-free liquid medium for the cultivation of some species of Neisseria. Bull World Health Organ. 1967;37(4):569–573. [PMC free article] [PubMed] [Google Scholar]
- SMITH R. T., THOMAS L. The lethal effect of endotoxins on the chick embryo. J Exp Med. 1956 Aug 1;104(2):217–231. doi: 10.1084/jem.104.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ueda K., Diena B. B., Greenberg L. The chick embryo neutralization test in the assay of meningococcal antibody. 1. Infection of the embryo with Neisseria meningitidis. Bull World Health Organ. 1969;40(2):235–240. [PMC free article] [PubMed] [Google Scholar]