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. 1984 Feb;49(2):333–342. doi: 10.1128/jvi.49.2.333-342.1984

Polyomavirus minichromosomes: associated DNA topoisomerase II and DNA ligase activities.

M R Krauss, B B Gourlie, M L Bayne, R M Benbow
PMCID: PMC255470  PMID: 6319733

Abstract

Polyomavirus minichromosomes were isolated and fractionated as described previously (B. B. Gourlie, M. R. Krauss, A. J. Buckler-White, R. M. Benbow, and V. Pigiet, J. Virol. 38:805-814, 1981). Specific assays for DNA topoisomerase II and DNA ligase activity were carried out on each fraction. The enzymatic activity in each fraction was determined by quantitative electron microscopy and compared with the number of replicative intermediate and total polyomavirus DNA molecules in each fraction. DNA topoisomerase II activity cosedimented with polyomavirus replicative intermediate minichromosomes. DNA ligase activity cosedimented with mature polyomavirus minichromosomes.

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Selected References

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