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. 1984 Feb;49(2):601–603. doi: 10.1128/jvi.49.2.601-603.1984

Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus.

B M Willumsen, R W Ellis, E M Scolnick, D R Lowy
PMCID: PMC255506  PMID: 6319763

Abstract

The sequences encoding the 21-kilodalton transforming protein (p21 ras) of Harvey murine sarcoma virus have previously been localized genetically to a 1.3-kilobase segment of the viral DNA (E. H. Chang, R. W. Ellis, E. M. Scolnick, and D. R. Lowy, Science 210:1249-1251, 1980). Within this segment, DNA sequence analysis has found a single open reading frame large enough to encode the viral p21 (R. Dhar, R. W. Ellis, T. Y. Shih, S. Oroszlan, B. Shapiro, J. Maizel, D. Lowy, and E. M. Scolnick, Science 217:934-937, 1982). There are three potential in-frame ATG initiation codons at the 5' end of this open reading frame. By constructing a mutant of Harvey murine sarcoma virus DNA from which the first two ATG codons of this open reading frame have been deleted, we now show by transfection of the mutant viral DNA into NIH 3T3 cells that only the third ATG codon is necessary and sufficient for synthesis of the viral p21 and for cellular transformation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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