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. 1984 Feb;49(2):621–625. doi: 10.1128/jvi.49.2.621-625.1984

Characterization of a metastable, partially replicated dimeric intermediate of minute virus of mice.

E A Faust, G Gloor
PMCID: PMC255510  PMID: 6319765

Abstract

Intracellular, replicative-form DNA of minute virus of mice was characterized by agarose gel electrophoresis, velocity sedimentation, electron microscopy, restriction endonuclease digestion, and sensitivity to the single-stranded nuclease S1. This analysis demonstrated the presence in murine cells infected with minute virus of mice of a 10.0-kilobase pair dimer replicative form, a 5-kilobase pair monomer replicative form, as well as a 5-kilobase viral single-stranded DNA species. Two additional viral DNA species that migrated in 0.5% agarose gels with apparent sizes of 8.0 and 5.5 kilobase pairs were also observed. Further investigation indicated that the 8.0-kilobase pair DNA represents a novel class of metastable, partially replicated, dimeric intermediates. This finding has important implications for the mechanism of parvovirus DNA replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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