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. 1984 Mar;49(3):674–679. doi: 10.1128/jvi.49.3.674-679.1984

DNA methylation inhibits the transfecting activity of replicative- form phi X174 DNA.

R Y Wang, S Shenoy, M Ehrlich
PMCID: PMC255523  PMID: 6321756

Abstract

Replacement of virtually all the cytosine residues with 5-methylcytosine residues in the complementary strand of the replicative form (RF) of phi X174 DNA caused a 300- to 500-fold loss in its transfecting activity. Similar results were obtained with analogously methylated M13 RF. Transfection experiments with phi X RF hemimethylated in only part of the molecule, as assessed by analysis with restriction endonucleases, indicated that gene A of phi X, which needs to be nicked at a specific site by the gene A protein for RF replication, was not the main target for this inhibition by DNA methylation. We propose that the loss of transfecting activity was due to hemimethylation of the phi X RF interfering with the processively catalyzed movement of the replication fork.

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Selected References

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