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. 1984 Mar;49(3):902–908. doi: 10.1128/jvi.49.3.902-908.1984

Isolation of the prohead core of bacteriophage T4 after cross-linking and determination of protein composition.

F Traub, B Keller, A Kuhn, M Maeder
PMCID: PMC255552  PMID: 6422052

Abstract

The naked core of bacteriophage T4 was isolated ex vivo after cross-linking with either glutaraldehyde or dithiobis(succinimidyl propionate). The isolated particles appeared to be morphologically identical to the cores found in thin sections, to those demonstrated in in situ lysis preparations, and to core structures assembled in vitro. Treatment with glutaraldehyde provided core particles which were morphologically well preserved, whereas dithiobis(succinimidyl propionate)-induced cross-linking was reversible and allowed analysis of the protein composition of the isolated particles. The identity of the reversibly cross-linked particles with those obtained after irreversible cross-linking was suggested by their morphology and their similar sedimentation behavior. Immunolabeling confirmed the structural presence of the main core protein in both structures. Gel electrophoresis of reversibly cross-linked cores revealed the essential head proteins gp22, gp67, and gp21, the three internal proteins IPI, IPII, and IPIII, and a 17K protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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