Abstract
Standardized methods are essential if phage typing of staphylococci is to be reliable and if the results obtained in different laboratories are to be compared. This paper, prepared on behalf of the Subcommittee on Phage Typing of Staphylococcus of the Nomenclature Committee of the International Association of Microbiological Societies, gives a detailed account of methods that have been found satisfactory for propagating the phages and defines a standard testing routine by which the stability of the phage preparations can be verified. Technical details are given of methods recommended for determining the phage type of staphylococci and the interpretation of differences between the types is discussed. The need for close collaboration between the epidemiologist and the laboratory worker in the use of phage typing is emphasized.
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Selected References
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- LIDWELL O. M. Apparatus for phagetyping of Staphylococcus aureus. Mon Bull Minist Health Public Health Lab Serv. 1959 Mar;18:49–52. [PubMed] [Google Scholar]
- LIU P. V. Cellophane plate technic applied to the production of bacteriophage. Am J Clin Pathol. 1958 Feb;29(2):176–180. doi: 10.1093/ajcp/29.2_ts.176. [DOI] [PubMed] [Google Scholar]
- SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
- TARR H. A. Mechanical aids for the phage-typing of Staphylococcus aureus. Mon Bull Minist Health Public Health Lab Serv. 1958 Mar;17:64–72. [PubMed] [Google Scholar]
- ZIERDT C. H. Preservation of staphylococcal bacteriophage by means of lyophilization. Am J Clin Pathol. 1959 Apr;31(4):326–331. doi: 10.1093/ajcp/31.4.326. [DOI] [PubMed] [Google Scholar]