Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1984 Mar;49(3):938–946. doi: 10.1128/jvi.49.3.938-946.1984

Herpesvirus saimiri DNA in a lymphoid cell line established by in vitro transformation.

S Schirm, I Müller, R C Desrosiers, B Fleckenstein
PMCID: PMC255556  PMID: 6321774

Abstract

A lymphoid T-cell line (H1591) was established by infecting peripheral blood mononuclear cells from a cotton top marmoset with Herpesvirus saimiri OMI. Analysis of these in vitro-immortalized cells revealed nonintegrated, covalently closed circular viral DNA molecules in high multiplicities with substantial rearrangements and large deletions in their L-DNA (unique) regions. One subline, designated H1591 Er, contained circular viral DNA with one stretch of H-DNA (repetitive) and one of L-DNA; the L-DNA segment consisted of a linear fusion of a 53.2-kilobase-pair piece of L-DNA (left half of L-DNA) with a 15.2-kilobase-pair L-DNA fragment from the right end of the L-DNA region. The other subline, H1591 S, contained two short regions of L-DNA, each derived from the extreme ends of virion L-DNA. Both L-DNA regions of H1591 S cells contained inverted repetitions (15.0 +/- 0.2 and 9.1 +/- 4.7 kilobase pairs). The extensive deletions of L-DNA sequences in cell line H1591 indicate that at least 73% of the genetic information in H. saimiri is not required to maintain the persistence of viral DNA and the state of transformation in lymphoid T-cells.

Full text

PDF
938

Images in this article

940Image
on p.940
940Image
on p.940
941Image
on p.941
943Image
on p.943
943Image
on p.943
944Image
on p.944
944Image
on p.944

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Da Silva M. P. Cuidados de enfermagem nos pacientes com "shunt" e fistula artério-venosa. Rev Enferm Nov Dimens. 1976 Nov;2(5):290–294. [PubMed] [Google Scholar]
  2. Deinhardt F. W., Falk L. A., Wolfe L. G. Simian herpesviruses and neoplasia. Adv Cancer Res. 1974;19(0):167–205. doi: 10.1016/s0065-230x(08)60054-8. [DOI] [PubMed] [Google Scholar]
  3. Desrosiers R. C. Herpesvirus saimiri DNA in tumor cells--deleted sequences and sequence rearrangements. J Virol. 1981 Aug;39(2):497–509. doi: 10.1128/jvi.39.2.497-509.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Desrosiers R. C. Specifically unmethylated cytidylic-guanylate sites in Herpesvirus saimiri DNA in tumor cells. J Virol. 1982 Aug;43(2):427–435. doi: 10.1128/jvi.43.2.427-435.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Falk L. A., Nigida S. M., Deinhardt F., Wolfe L. G., Cooper R. W., Hernandez-Camacho J. I. Herpesvirus ateles: properties of an oncogenic herpesvirus isolated from circulating lymphocytes of spider monkeys (Ateles sp.). Int J Cancer. 1974 Oct 15;14(4):473–482. doi: 10.1002/ijc.2910140407. [DOI] [PubMed] [Google Scholar]
  6. Falk L. A., Wolfe L. G., Deinhardt F. Isolation of Herpesvirus saimiri from blood of squirrel monkeys (Saimiri sciureus). J Natl Cancer Inst. 1972 May;48(5):1499–1505. [PubMed] [Google Scholar]
  7. Falk L., Johnson D., Deinhardt F. Transformation of marmoset lymphocytes in vitro with Herpesvirus ateles. Int J Cancer. 1978 May 15;21(5):652–657. doi: 10.1002/ijc.2910210517. [DOI] [PubMed] [Google Scholar]
  8. Falk L., Wright J., Wolfe L., Deinhardt F. Herpesvirus ateles: transformation in vitro of marmoset splenic lymphocytes. Int J Cancer. 1974 Aug 15;14(2):244–251. doi: 10.1002/ijc.2910140213. [DOI] [PubMed] [Google Scholar]
  9. Fleckenstein B., Bornkamm G. W., Ludwig H. Repetitive sequences in complete and defective genomes of Herpesvirus saimiri. J Virol. 1975 Feb;15(2):398–406. doi: 10.1128/jvi.15.2.398-406.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fleckenstein B., Müller I., Werner J. The presence of Herpesvirus Saimiri genomes in virus-transformed cells. Int J Cancer. 1977 Apr 15;19(4):546–554. doi: 10.1002/ijc.2910190416. [DOI] [PubMed] [Google Scholar]
  11. Fleckenstein B. Oncogenic herpesviruses of non-human primates. Biochim Biophys Acta. 1979 Nov 30;560(3):301–342. doi: 10.1016/0304-419x(79)90007-6. [DOI] [PubMed] [Google Scholar]
  12. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  13. Hunt R. D., Garcia F. G., Barahona H. H., King N. W., Fraser C. E., Meléndez L. V. Spontaneous Herpesvirus saimiri lymphoma in an owl monkey. J Infect Dis. 1973 Jun;127(6):723–725. doi: 10.1093/infdis/127.6.723. [DOI] [PubMed] [Google Scholar]
  14. Kaschka-Dierich C., Werner F. J., Bauer I., Fleckenstein B. Structure of nonintegrated, circular Herpesvirus saimiri and Herpesvirus ateles genomes in tumor cell lines and in vitro-transformed cells. J Virol. 1982 Oct;44(1):295–310. doi: 10.1128/jvi.44.1.295-310.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Knust E., Dietrich W., Fleckenstein B., Bodemer W. Virus-specific transcription in a Herpesvirus saimiri-transformed lymphoid tumor cell line. J Virol. 1983 Nov;48(2):377–383. doi: 10.1128/jvi.48.2.377-383.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knust E., Schirm S., Dietrich W., Bodemer W., Kolb E., Fleckenstein B. Cloning of Herpesvirus saimiri DNA fragments representing the entire L-region of the genome. Gene. 1983 Nov;25(2-3):281–289. doi: 10.1016/0378-1119(83)90232-9. [DOI] [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  20. Todaro G. J., Sherr C. J., Sen A., King N., Daniel M. D., Fleckenstein B. Endogenous New World primate type C viruses isolated from owl monkey (Aotus trivirgatus) kidney cell line. Proc Natl Acad Sci U S A. 1978 Feb;75(2):1004–1008. doi: 10.1073/pnas.75.2.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wallen W. C., Neubauer R. H., Rabin H., Cicmanec J. L. Nonimmune rosette formation by lymphoma and leukemia cells from Herpesvirus saimiri-infected owl monkeys. J Natl Cancer Inst. 1973 Sep;51(3):967–975. doi: 10.1093/jnci/51.3.967. [DOI] [PubMed] [Google Scholar]
  23. Werner F. J., Bornkamm G. W., Fleckenstein B. Episomal viral DNA in a Herpesvirus saimiri-transformed lymphoid cell line. J Virol. 1977 Jun;22(3):794–803. doi: 10.1128/jvi.22.3.794-803.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES