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. 1984 Apr;50(1):60–65. doi: 10.1128/jvi.50.1.60-65.1984

Asymmetric transcription of mouse mammary tumor virus genes in vivo and in vitro.

R S Prewitt, L D Washington, M R Stallcup
PMCID: PMC255582  PMID: 6321800

Abstract

Mouse mammary tumor virus (MMTV) DNA fragments were cloned into M13 bacteriophage, and the single-stranded recombinant phage DNAs were used as strand-specific nucleic acid hybridization probes to measure synthesis of plus (genomic) and minus strands of MMTV RNA in cultured cell lines and in cell-free preparations of nuclei. Pulse-labeling studies showed that synthesis of MMTV RNA in three different cell lines was highly asymmetric. In nuclear preparations from a cloned line of MMTV-infected rat hepatoma cells, elongation of nascent MMTV RNA chains and initiation of new MMTV RNA chains with nucleoside (beta-S)triphosphates were also highly asymmetric.

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Selected References

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