Abstract
We isolated DNA clones of intracisternal A-particle (IAP) genes from the genome of an Asian wild mouse, Mus caroli. A typical M. caroli IAP gene was 6.5 kilobase pairs in length and had long terminal repeat (LTR) sequences at both ends. The size of the LTR was 345 base pairs in clone L20, and two LTRs at both ends of this clone were linked to directly repeating cellular sequences of 6 base pairs. Each LTR possessed most of the structural features commonly associated with the retrovirus LTR. The restriction map of the M. caroli IAP gene resembled that of Mus musculus, although the M. caroli IAP gene was 0.4 kilobase pairs shorter than the M. musculus IAP gene in two regions. Sequence homology between the M. caroli and M. musculus IAP LTRs was calculated as about 80%, whereas the LTR sequence of the Syrian hamster IAP gene was about 60% homologous to the M. caroli LTR. The reiteration frequency of the M. caroli IAP genes was estimated as 200 to 400 copies per haploid genome, which is at least 10 times the reported value. These results suggest that the IAP genes observed in the genus Mus are present in multiple copies with structures closely resembling the integrated retrovirus gene.
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Selected References
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- Biczysko W., Pienkowski M., Solter D., Koprowski H. Virus particles in early mouse embryos. J Natl Cancer Inst. 1973 Sep;51(3):1041–1050. doi: 10.1093/jnci/51.3.1041. [DOI] [PubMed] [Google Scholar]
- Calarco P. G., Szollosi D. Intracisternal A particles in ova and preimplantation stages of the mouse. Nat New Biol. 1973 May 16;243(124):91–93. [PubMed] [Google Scholar]
- Callahan R., Kuff E. L., Lueders K. K., Birkenmeier E. Genetic relationship between the Mus cervicolor M432 retrovirus and the Mus Musculus intracisternal type A particle. J Virol. 1981 Dec;40(3):901–911. doi: 10.1128/jvi.40.3.901-911.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase D. G., Pikó L. Expression of A- and C-type particles in early mouse embryos. J Natl Cancer Inst. 1973 Dec;51(6):1971–1975. doi: 10.1093/jnci/51.6.1971. [DOI] [PubMed] [Google Scholar]
- Cole M. D., Ono M., Huang R. C. Intracisternal A-particle genes: structure of adjacent genes and mapping of the boundaries of the transcriptional unit. J Virol. 1982 Apr;42(1):123–130. doi: 10.1128/jvi.42.1.123-130.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cole M. D., Ono M., Huang R. C. Terminally redundant sequences in cellular intracisternal A-particle genes. J Virol. 1981 May;38(2):680–687. doi: 10.1128/jvi.38.2.680-687.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall W. T., Hartley J. W., Sanford K. K. Characteristics of and relationship between C particles and intracisternal A particles in cloned cell strains. J Virol. 1968 Mar;2(3):238–247. doi: 10.1128/jvi.2.3.238-247.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hawley R. G., Shulman M. J., Murialdo H., Gibson D. M., Hozumi N. Mutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7425–7429. doi: 10.1073/pnas.79.23.7425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuff E. L., Feenstra A., Lueders K., Rechavi G., Givol D., Canaani E. Homology between an endogenous viral LTR and sequences inserted in an activated cellular oncogene. Nature. 1983 Apr 7;302(5908):547–548. doi: 10.1038/302547a0. [DOI] [PubMed] [Google Scholar]
- Kuff E. L., Feenstra A., Lueders K., Smith L., Hawley R., Hozumi N., Shulman M. Intracisternal A-particle genes as movable elements in the mouse genome. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1992–1996. doi: 10.1073/pnas.80.7.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuff E. L., Lueders K. K., Scolnick E. M. Nucleotide sequence relationship between intracisternal type A particles of Mus musculus and an endogenous retrovirus (M432) of Mus cervicolor. J Virol. 1978 Oct;28(1):66–74. doi: 10.1128/jvi.28.1.66-74.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuff E. L., Smith L. A., Lueders K. K. Intracisternal A-particle genes in Mus musculus: a conserved family of retrovirus-like elements. Mol Cell Biol. 1981 Mar;1(3):216–227. doi: 10.1128/mcb.1.3.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuff E. L., Wivel N. A., Lueders K. K. The extraction of intracisternal A-particles from a mouse plasma-cell tumor. Cancer Res. 1968 Oct;28(10):2137–2148. [PubMed] [Google Scholar]
- Lueders K. K., Kuff E. L. Comparison of the sequence organization of related retrovirus-like multigene families in three evolutionarily distant rodent genomes. Nucleic Acids Res. 1983 Jul 11;11(13):4391–4408. doi: 10.1093/nar/11.13.4391. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lueders K. K., Kuff E. L. Genetic individuality of intracisternal A-particles of Mus musculus. J Virol. 1979 Apr;30(1):225–231. doi: 10.1128/jvi.30.1.225-231.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lueders K. K., Kuff E. L. Intracisternal A-particle genes: identification in the genome of Mus musculus and comparison of multiple isolates from a mouse gene library. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3571–3575. doi: 10.1073/pnas.77.6.3571. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lueders K. K., Kuff E. L. Sequences associated with intracisternal A particles are reiterated in the mouse genome. Cell. 1977 Dec;12(4):963–972. doi: 10.1016/0092-8674(77)90161-1. [DOI] [PubMed] [Google Scholar]
- Lueders K. K., Kuff E. L. Sequences homologous to retrovirus-like genes of the mouse are present in multiple copies in the Syrian hamster genome. Nucleic Acids Res. 1981 Nov 25;9(22):5917–5930. doi: 10.1093/nar/9.22.5917. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ono M., Cole M. D., White A. T., Huang R. C. Sequence organization of cloned intracisternal A particle genes. Cell. 1980 Sep;21(2):465–473. doi: 10.1016/0092-8674(80)90483-3. [DOI] [PubMed] [Google Scholar]
- Ono M., Ohishi H. Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNAPhe as a putative primer tRNA. Nucleic Acids Res. 1983 Oct 25;11(20):7169–7179. doi: 10.1093/nar/11.20.7169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paterson B. M., Segal S., Lueders K. K., Kuff E. L. RNA associated with murine intracisternal type A particles codes for the main particle protein. J Virol. 1978 Jul;27(1):118–126. doi: 10.1128/jvi.27.1.118-126.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rechavi G., Givol D., Canaani E. Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like element. Nature. 1982 Dec 16;300(5893):607–611. doi: 10.1038/300607a0. [DOI] [PubMed] [Google Scholar]
- Shen-Ong G. L., Cole M. D. Differing populations of intracisternal A-particle genes in myeloma tumors and mouse subspecies. J Virol. 1982 May;42(2):411–421. doi: 10.1128/jvi.42.2.411-421.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki A., Kitasato H., Kawakami M., Ono M. Molecular cloning of retrovirus-like genes present in multiple copies in the Syrian hamster genome. Nucleic Acids Res. 1982 Oct 11;10(19):5733–5746. doi: 10.1093/nar/10.19.5733. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Temin H. M. Structure, variation and synthesis of retrovirus long terminal repeat. Cell. 1981 Nov;27(1 Pt 2):1–3. doi: 10.1016/0092-8674(81)90353-6. [DOI] [PubMed] [Google Scholar]
- Tu C. P., Cohen S. N. 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Gene. 1980 Jul;10(2):177–183. doi: 10.1016/0378-1119(80)90135-3. [DOI] [PubMed] [Google Scholar]
- Wilson S. H., Kuff E. L. A novel DNA polymerase activity found in association with intracisternal A-type particles. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1531–1536. doi: 10.1073/pnas.69.6.1531. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wivel N. A., Smith G. H. Distribution of intracisternal A-particles in a variety of normal and neoplastic mouse tissues. Int J Cancer. 1971 Jan 15;7(1):167–175. doi: 10.1002/ijc.2910070119. [DOI] [PubMed] [Google Scholar]