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Thermodynamic Stabilitiesa of wt PLRV pk-RNA and Selected Mutants
| Sequence | Efficiency [%]a | Tm [°C] | |
|---|---|---|---|
| wta | 8.8 | 50 |
|
| C17U/A18C | 18.7 (2.12) | 62 | |
| ΔU12 | 0.7 (0.08) | 42 | |
| ΔA18-A21 | 8.6 (0.98) | 38 |
The graph on the right depicts molar ellipticities vs. temperature
Efficiency relative to wt shown in parentheses
Sequence 5’-GCG GCA CCG UCC GCC AAA ACA AAC GG (U12 and C17-A21 underlined)
