Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1984 May;50(2):380–386. doi: 10.1128/jvi.50.2.380-386.1984

Cell-mediated cytotoxicity against targets bearing Sendai virus glycoproteins in the absence of viral infection.

T F Abidi, T D Flanagan
PMCID: PMC255631  PMID: 6323738

Abstract

The glycoproteins HN and F and the lipids were solubilized from Sendai virus envelopes by using the nonionic detergent beta-D-octylglucoside. When beta-D-octylglucoside was removed by dialysis, the glycoproteins and lipids reassociated to form vesicles. These vesicles displayed hemagglutinating, neuraminidase, and hemolysin activities comparable to those expressed by the intact virus. The vesicles were used as carriers to transfer the glycoproteins to the surface of P815 cells. The recipient cells were tested for the acquisition of the glycoproteins by demonstration of surface neuraminidase, hemadsorption activity, and antigens. The modified cells were used as targets for natural cell-mediated lysis and were found to be sensitive.

Full text

PDF
380

Images in this article

382Image
on p.382
383Image
on p.383
384Image
on p.384
384Image
on p.384

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson M. J. Innate cytotoxicity of CBA mouse spleen cells to Sendai virus-infected L cells. Infect Immun. 1978 Jun;20(3):608–612. doi: 10.1128/iai.20.3.608-612.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop G. A., Glorioso J. C., Schwartz S. A. Role of interferon in human natural killer activity against target cells infected with HSV-1. J Immunol. 1983 Oct;131(4):1849–1853. [PubMed] [Google Scholar]
  4. Casali P., Sissons J. G., Buchmeier M. J., Oldstone M. B. In vitro generation of human cytotoxic lymphocytes by virus. Viral glycoproteins induce nonspecific cell-mediated cytotoxicity without release of interferon. J Exp Med. 1981 Sep 1;154(3):840–855. doi: 10.1084/jem.154.3.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Choppin P. W., Scheid A. The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis. 1980 Jan-Feb;2(1):40–61. doi: 10.1093/clinids/2.1.40. [DOI] [PubMed] [Google Scholar]
  6. Fitzgerald P. A., von Wussow P., Lopez C. Role of interferon in natural kill of HSV-1-infected fibroblasts. J Immunol. 1982 Aug;129(2):819–823. [PubMed] [Google Scholar]
  7. Haywood A. M. Letter to the editor: Fusion of Sendai viruses with model membranes. J Mol Biol. 1974 Aug 15;87(3):625–628. doi: 10.1016/0022-2836(74)90107-7. [DOI] [PubMed] [Google Scholar]
  8. Helenius A., Sarvas M., Simons K. Asymmetric and symmetric membrane reconstitution by detergent elimination. Studies with Semliki-Forest-virus spike glycoprotein and penicillinase from the membrane of Bacillus licheniformis. Eur J Biochem. 1981 May;116(1):27–35. doi: 10.1111/j.1432-1033.1981.tb05296.x. [DOI] [PubMed] [Google Scholar]
  9. Hosaka Y., Shimizu Y. K. Artificial assembly of envelope particles of HVJ (Sendai virus). I. Assembly of hemolytic and fusion factors from envelopes solubilized by Nonidet P40. Virology. 1972 Sep;49(3):627–639. doi: 10.1016/0042-6822(72)90519-3. [DOI] [PubMed] [Google Scholar]
  10. Hsu M. C., Scheid A., Choppin P. W. Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution. Virology. 1979 Jun;95(2):476–491. doi: 10.1016/0042-6822(79)90502-6. [DOI] [PubMed] [Google Scholar]
  11. Huang R. T., Wahn K., Klenk H. D., Rott R. Association of the envelope glycoproteins of influenza virus with liposomes--a model study on viral envelope assembly. Virology. 1979 Aug;97(1):212–217. doi: 10.1016/0042-6822(79)90390-8. [DOI] [PubMed] [Google Scholar]
  12. Härfast B., Andersson T., Perlmann P. Immunoglobulin-independent natural cytotoxicity of Fc receptor-bearing human blood lymphocytes to mumps virus-infected target cells. J Immunol. 1978 Aug;121(2):755–761. [PubMed] [Google Scholar]
  13. Ito Y., Hosaka Y. Component(s) of Sendai virus that can induce interferon in mouse spleen cells. Infect Immun. 1983 Mar;39(3):1019–1023. doi: 10.1128/iai.39.3.1019-1023.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Koszinowski U., Gething M. J., Waterfield M. T-cell cytotoxicity in the absence of viral protein synthesis in target cells. Nature. 1977 May 12;267(5607):160–163. doi: 10.1038/267160a0. [DOI] [PubMed] [Google Scholar]
  15. Lundstedt C. Effect of antilymphocyte serum on adoptive immunization of lymphocytic choriomeningitis virus carrier mice. Acta Pathol Microbiol Scand. 1969;77(3):518–526. doi: 10.1111/j.1699-0463.1969.tb04258.x. [DOI] [PubMed] [Google Scholar]
  16. Minato N., Bloom B. R., Jones C., Holland J., Reid L. M. Mechanism of rejection of virus persistently infected tumor cells by athymic nude mice. J Exp Med. 1979 May 1;149(5):1117–1133. doi: 10.1084/jem.149.5.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Patzer E. J., Wagner R. R., Dubovi E. J. Viral membranes: model systems for studying biological membranes. CRC Crit Rev Biochem. 1979;6(2):165–217. doi: 10.3109/10409237909102563. [DOI] [PubMed] [Google Scholar]
  18. Petri W. A., Jr, Wagner R. R. Reconstitution into liposomes of the glycoprotein of vesicular stomatitis virus by detergent dialysis. J Biol Chem. 1979 Jun 10;254(11):4313–4316. [PubMed] [Google Scholar]
  19. Roder J. C., Rosén A., Fenyö E. M., Troy F. A. Target-effector interaction in the natural killer cell system: isolation of target structures. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1405–1409. doi: 10.1073/pnas.76.3.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Santoli D., Trinchieri G., Koprowski H. Cell-mediated cytotoxicity against virus-infected target cells in humans. II. Interferon induction and activation of natural killer cells. J Immunol. 1978 Aug;121(2):532–538. [PubMed] [Google Scholar]
  21. Shearer G. M., Garbarino C. A., Cudkowicz G. In vitro induction of F1 hybrid anti-parent cell-mediated cytotoxicity. J Immunol. 1976 Sep;117(3):754–759. [PubMed] [Google Scholar]
  22. Shimizu K., Shimizu Y. K., Koama T., Ishida N. Isolation and characterization of two distinct types of HVJ (Sendai virus) spikes. Virology. 1974 Nov;62(1):90–101. doi: 10.1016/0042-6822(74)90305-5. [DOI] [PubMed] [Google Scholar]
  23. Smith H. G., Jr, Stubbs G. W., Litman B. J. The isolation and purification of osmotically intact discs from retinal rod outer segments. Exp Eye Res. 1975 Mar;20(3):211–217. doi: 10.1016/0014-4835(75)90134-7. [DOI] [PubMed] [Google Scholar]
  24. Sugamura K., Shimizu K., Zarling D. A., Bach F. H. Role of sendai virus fusion-glycoprotein in target cell susceptibility to cytotoxic T cells. Nature. 1977 Nov 17;270(5634):251–253. doi: 10.1038/270251a0. [DOI] [PubMed] [Google Scholar]
  25. Welsh R. M., Jr, Hallenbeck L. A. Effect of virus infections on target cell susceptibility to natural killer cell-mediated lysis. J Immunol. 1980 May;124(5):2491–2497. [PubMed] [Google Scholar]
  26. Yap K. L., Ada G. L. Cytotoxic T cells specific for influenza virus-infected target cells. Immunology. 1977 Feb;32(2):151–159. [PMC free article] [PubMed] [Google Scholar]
  27. Zinkernagel R. M., Doherty P. C. H-2 compatability requirement for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. Different cytotoxic T-cell specificities are associated with structures coded for in H-2K or H-2D;. J Exp Med. 1975 Jun 1;141(6):1427–1436. doi: 10.1084/jem.141.6.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES