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. 1984 May;50(2):408–416. doi: 10.1128/jvi.50.2.408-416.1984

Tumorigenic poxviruses: construction of the composite physical map of the Shope fibroma virus genome.

A M Delange, C Macaulay, W Block, T Mueller, G McFadden
PMCID: PMC255634  PMID: 6323741

Abstract

The sites for the restriction enzymes BamHI, Bg/I, HindIII, PstI, PvuII, and SstI on the linear DNA genome of Shope fibroma virus, a tumorigenic poxvirus of rabbits, have been determined by digestions of the cloned BamHI and HindIII restriction fragments and by hybridization of 32P-labeled cloned fragments to Southern blots of Shope fibroma virus DNA cleaved partially or completely with the various enzymes. The linear genome is shown to be 160 kilobases in length and to possess terminal inverted repeat sequences of between 12.2 and 12.5 kilobases extending inwards from the cross-linked DNA telomeres. The fine map of the Shope fibroma virus terminal inverted repeats has been constructed and shown to be distinctly different from that of members of the orthopoxvirus group, such as vaccinia, by the absence of detectable tandemly repeated sequences near the termini and by the lack of detectable sequence homology with vaccinia termini.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baroudy B. M., Moss B. Sequence homologies of diverse length tandem repetitions near ends of vaccinia virus genome suggest unequal crossing over. Nucleic Acids Res. 1982 Sep 25;10(18):5673–5679. doi: 10.1093/nar/10.18.5673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baroudy B. M., Venkatesan S., Moss B. Incompletely base-paired flip-flop terminal loops link the two DNA strands of the vaccinia virus genome into one uninterrupted polynucleotide chain. Cell. 1982 Feb;28(2):315–324. doi: 10.1016/0092-8674(82)90349-x. [DOI] [PubMed] [Google Scholar]
  3. Baroudy B. M., Venkatesan S., Moss B. Structure and replication of vaccinia virus telomeres. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):723–729. doi: 10.1101/sqb.1983.047.01.083. [DOI] [PubMed] [Google Scholar]
  4. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Garon C. F., Barbosa E., Moss B. Visualization of an inverted terminal repetition in vaccinia virus DNA. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4863–4867. doi: 10.1073/pnas.75.10.4863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Godson G. N., Vapnek D. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA. Biochim Biophys Acta. 1973 Apr 11;299(4):516–520. doi: 10.1016/0005-2787(73)90223-2. [DOI] [PubMed] [Google Scholar]
  7. Holowczak J. A. Poxvirus DNA. Curr Top Microbiol Immunol. 1982;97:27–79. doi: 10.1007/978-3-642-68318-3_2. [DOI] [PubMed] [Google Scholar]
  8. Jacquemont B., Grange J., Gazzolo L., Richard M. H. Composition and size of Shope fibroma virus deoxyribonucleic acid. J Virol. 1972 May;9(5):836–841. doi: 10.1128/jvi.9.5.836-841.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Langridge J., Langridge P., Bergquist P. L. Extraction of nucleic acids from agarose gels. Anal Biochem. 1980 Apr;103(2):264–271. doi: 10.1016/0003-2697(80)90266-3. [DOI] [PubMed] [Google Scholar]
  10. McFadden G., Dales S. Biogenesis of poxviruses: mirror-image deletions in vaccinia virus DNA. Cell. 1979 Sep;18(1):101–108. doi: 10.1016/0092-8674(79)90358-1. [DOI] [PubMed] [Google Scholar]
  11. Moss B., Winters E., Cooper N. Instability and reiteration of DNA sequences within the vaccinia virus genome. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1614–1618. doi: 10.1073/pnas.78.3.1614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pickup D. J., Bastia D., Stone H. O., Joklik W. K. Sequence of terminal regions of cowpox virus DNA: arrangement of repeated and unique sequence elements. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7112–7116. doi: 10.1073/pnas.79.23.7112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  14. Rosenberg H., Singer M., Rosenberg M. Highly reiterated sequences of SIMIANSIMIANSIMIANSIMIANSIMIAN. Science. 1978 Apr 28;200(4340):394–402. doi: 10.1126/science.205944. [DOI] [PubMed] [Google Scholar]
  15. Sell S., Scott C. B. An immunohistologic study of Shope fibroma virus in rabbits: tumor rejection by cellular reaction in adults and progressive systemic reticuloendothelial infection in neonates. J Natl Cancer Inst. 1981 Feb;66(2):363–373. [PubMed] [Google Scholar]
  16. Singer D. S. Arrangement of a highly repeated DNA sequence in the genome and chromatin of the African green monkey. J Biol Chem. 1979 Jun 25;254(12):5506–5514. [PubMed] [Google Scholar]
  17. Smith J. W., Tevethia S. S., Levy B. M., Rawls W. E. Comparative studies on host responses to Shope fibroma virus in adult and newborn rabbits. J Natl Cancer Inst. 1973 Jun;50(6):1529–1539. doi: 10.1093/jnci/50.6.1529. [DOI] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weir J. P., Moss B. Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations. J Virol. 1983 May;46(2):530–537. doi: 10.1128/jvi.46.2.530-537.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wills A., Delange A. M., Gregson C., Macaulay C., McFadden G. Physical characterization and molecular cloning of the Shope fibroma virus DNA genome. Virology. 1983 Oct 30;130(2):403–414. doi: 10.1016/0042-6822(83)90095-8. [DOI] [PubMed] [Google Scholar]
  22. Wittek R., Moss B. Tandem repeats within the inverted terminal repetition of vaccinia virus DNA. Cell. 1980 Aug;21(1):277–284. doi: 10.1016/0092-8674(80)90135-x. [DOI] [PubMed] [Google Scholar]
  23. Wittek R., Müller H. K., Wyler R. Length heterogeneity in the DNA of vaccinia virus is eliminated on cloning the virus. FEBS Lett. 1978 Jun 1;90(1):41–46. doi: 10.1016/0014-5793(78)80293-2. [DOI] [PubMed] [Google Scholar]
  24. Wittek R. Organization and expression of the poxvirus genome. Experientia. 1982 Mar 15;38(3):285–297. doi: 10.1007/BF01949349. [DOI] [PubMed] [Google Scholar]

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