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. 2008 Jul 23;112(7):2761–2769. doi: 10.1182/blood-2008-02-142158

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© 2008 by The American Society of Hematology

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Activity decay of WT and mutant factor VIII. Factor VIII (4 nM) was incubated at various temperatures (52°C-60°C); and at the indicated times, aliquots were removed and assayed for activity by factor Xa generation assays. Data were fitted by nonlinear least squares regression, and decay rates were obtained. Each point represents the value averaged from 3 separate determinations. Results are shown for WT (, ×), Glu272Ala (□), Glu272Val (■), Asp519Ala (○), Asp519Val (●), Glu665Ala (△), Glu665Val (▲), Glu1984Ala (◇), Glu1984Val (♦), and full-length Kogenate factor VIII (). (A) Representative factor VIII decay curves after 55°C incubation. (B) Plots of factor VIII decay rate at various temperatures. (Inset) Magnified view of the decay results incubated at 52°C to 55°C.

Inline graphic — Activity decay of WT and mutant factor VIII. Factor VIII (4 nM) was incubated at various temperatures (52°C-60°C); and at the indicated times, aliquots were removed and assayed for activity by factor Xa generation assays. Data were fitted by nonlinear least squares regression, and decay rates were obtained. Each point represents the value averaged from 3 separate determinations. Results are shown for WT (, ×), Glu272Ala (□), Glu272Val (■), Asp519Ala (○), Asp519Val (●), Glu665Ala (△), Glu665Val (▲), Glu1984Ala (◇), Glu1984Val (♦), and full-length Kogenate factor VIII (). (A) Representative factor VIII decay curves after 55°C incubation. (B) Plots of factor VIII decay rate at various temperatures. (Inset) Magnified view of the decay results incubated at 52°C to 55°C.