Table 1.
Denileukin diftitox (DAB389IL-2) treatment reduced HIV-1 infection in DKO-hu HSC mice
| Donor | Mice no. | Treatment | Organ | Percentage cell population |
Absolute cell number per organ |
Plasma viral load, copies/mL |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD45+* | CD3+† | CD4+‡ | CD8+‡ | Foxp3+§ | p24+§ | CD45+ | CD3+ | CD4+CD8− | CD8+CD4− | Foxp3+ | Day 4‖ | Day 7 | Day 10 | ||||
| 1 | M56 | Mock | Spleen | 11.7 | 27.5 | 66 | 29.4 | 2.22 | 0.09 | 7.5E+06 | 2.1E+06 | 1.4E+06 | 1.5E+06 | 3.2E+04 | ND | 0 | 0 |
| 1 | M58 | PBS, HIV-1 | Spleen | 14.2 | 17.7 | 66.5 | 27.5 | 0.59 | 11.4 | 2.5E+05 | 4.4E+04 | 2.9E+04 | 3.2E+04 | 1.9E+02 | ND | 5.5E+03 | 1.1E+05 |
| 1 | M59 | PBS, HIV-1 | Spleen | 15.6 | 15 | 55 | 36.7 | 1.24 | 37.7 | 8.1E+06 | 1.2E+06 | 6.7E+05 | 7.7E+05 | 9.5E+03 | ND | 2.3E+04 | 8.1E+04 |
| 1 | M54 | DD, HIV-1 | Spleen | 26.1 | 18.9 | 51 | 43.3 | 2.64 | 0.23 | 1.9E+07 | 3.6E+06 | 1.9E+06 | 2.1E+06 | 5.4E+04 | ND | ND | ND |
| 1 | M57 | DD, HIV-1 | Spleen | 21.2 | 19.3 | 40 | 39.2 | 1.62 | 4.3 | 2.4E+07 | 4.7E+06 | 1.9E+06 | 2.9E+06 | 4.7E+04 | ND | 5.8E+03 | ND |
| 1 | M56 | Mock | mLN | 86.2 | 58.7 | 68.4 | 26.7 | 3 | 0.02 | 2.1E+06 | 1.2E+06 | 8.3E+05 | 8.8E+05 | 2.7E+04 | — | — | — |
| 1 | M58 | PBS, HIV-1 | mLN | 83.8 | 52.5 | 67.1 | 29.8 | 0.72 | 47.5 | 8.4E+05 | 4.4E+05 | 2.9E+05 | 3.1E+05 | 2.2E+03 | — | — | — |
| 1 | M59 | PBS, HIV-1 | mLN | 85.2 | 23.8 | 32.5 | 62 | 0.85 | 78.1 | 1.4E+06 | 3.2E+05 | 1.1E+05 | 1.2E+05 | 1.0E+03 | — | — | — |
| 1 | M54 | DD, HIV-1 | mLN | 95.4 | 72 | 57.1 | 40.9 | 1.71 | 0 | 3.6E+06 | 2.6E+06 | 1.5E+06 | 1.5E+06 | 2.6E+04 | — | — | — |
| 1 | M57 | DD, HIV-1 | mLN | 93.3 | 67.7 | 57.4 | 38.6 | 0.3 | 12.64 | 3.6E+06 | 2.5E+06 | 1.4E+06 | 1.5E+06 | 4.5E+03 | — | — | — |
| 2 | 582 | Mock | Spleen | 2.4 | 54.1 | 77.9 | 18.5 | 3.37 | 0.35 | 2.3E+06 | 1.2E+06 | 9.7E+05 | 1.0E+06 | 3.4E+04 | ND | ND | ND |
| 2 | 580 | Mock | Spleen | 12.0 | 58.6 | 68.2 | 27.1 | 11.07 | 0.18 | 4.9E+06 | 2.9E+06 | 2.0E+06 | 2.1E+06 | 2.3E+05 | ND | ND | ND |
| 2 | 576 | PBS, HIV-1 | Spleen | 1.4 | 17.9 | 4.1 | 84.2 | 0.21 | 23.1 | 1.2E+06 | 2.2E+05 | 9.0E+03 | 3.5E+04 | 7.3E+01 | 6.1E+04 | 1.9E+05 | 2.2E+05 |
| 2 | 583 | PBS, HIV-1 | Spleen | 1.4 | 45.1 | 5.2 | 84.8 | 0.32 | 22.4 | 2.0E+06 | 9.1E+05 | 4.7E+04 | 1.4E+05 | 4.4E+02 | ND | 5.7E+04 | 9.3E+04 |
| 2 | 577 | DD, HIV-1 | Spleen | 5.1 | 9.8 | 34.6 | 53.1 | 0.52 | 47.5 | 4.8E+06 | 4.7E+05 | 1.6E+05 | 2.2E+05 | 1.1E+03 | ND | 8.2E+04 | 1.7E+05 |
| 2 | 579 | DD, HIV-1 | Spleen | 5.4 | 29 | 70.2 | 22.5 | 0.42 | 14.77 | 3.5E+06 | 1.0E+06 | 7.2E+05 | 8.0E+05 | 3.3E+03 | 3.1E+03 | 2.3E+04 | 6.6E+04 |
| 2 | 582 | Mock | mLN | 72.5 | 72.3 | 62.5 | 33.5 | 1.22 | 0.04 | 6.7E+05 | 4.8E+05 | 3.0E+05 | 3.2E+05 | 3.9E+03 | — | — | — |
| 2 | 580 | Mock | mLN | 91.5 | 81.9 | 67.5 | 29.3 | 4.82 | 0.02 | 3.2E+06 | 2.6E+06 | 1.8E+06 | 1.9E+06 | 9.0E+04 | — | — | — |
| 2 | 576 | PBS, HIV-1 | mLN | 77.1 | 50.7 | 0.37 | 94.5 | 1 | 8.7 | 4.8E+05 | 2.5E+05 | 9.1E+02 | 1.4E+04 | 1.4E+02 | — | — | — |
| 2 | 583 | PBS, HIV-1 | mLN | 56.5 | 98.2 | 14.8 | 78.5 | 2.6 | 70.9 | 1.8E+05 | 1.8E+05 | 2.6E+04 | 3.8E+04 | 9.8E+02 | — | — | — |
| 2 | 577 | DD, HIV-1 | mLN | 75.4 | 37.7 | 16.6 | 78.2 | 0.91 | 71.1 | 5.0E+05 | 1.9E+05 | 3.1E+04 | 4.1E+04 | 3.7E+02 | — | — | — |
| 2 | 579 | DD, HIV-1 | mLN | 87.6 | 71.1 | 60.6 | 35 | 0.22 | 57 | 1.3E+06 | 9.0E+05 | 5.5E+05 | 5.9E+05 | 1.3E+03 | — | — | — |
| 3 | 454 | PBS, mock | mLN | 14.6 | 60.7 | 83.3 | 10.8 | 8.43 | 0.2 | 7.6E+03 | 4.6E+03 | 3.9E+03 | 5.0E+02 | 3.9E+02 | — | ND | — |
| 3 | 463 | PBS, mock | mLN | 67.9 | 63.2 | 72.6 | 21.5 | 9.77 | 0.23 | 1.3E+05 | 8.2E+04 | 5.9E+04 | 1.8E+04 | 8.0E+03 | — | ND | — |
| 3 | 461 | DD, mock | mLN | 93.5 | 64.3 | 78.2 | 19.5 | 1.49 | 0.05 | 1.1E+06 | 6.8E+05 | 5.4E+05 | 1.3E+05 | 1.0E+04 | — | ND | — |
| 3 | 462 | DD, mock | mLN | 79.0 | 49.1 | 75.9 | 21.6 | 1.15 | 0.33 | 3.8E+05 | 1.9E+05 | 1.4E+05 | 4.0E+04 | 2.1E+03 | — | ND | — |
| 3 | 457 | PBS, HIV-1 | mLN | 27.1 | 51.9 | 67.1 | 29.8 | 5.33 | 1.35 | 7.3E+03 | 3.8E+03 | 2.5E+03 | 1.1E+03 | 2.0E+02 | — | 9.3E+05 | — |
| 3 | 460 | PBS, HIV-1 | mLN | 83.2 | 65.8 | 76.9 | 19.8 | 4.23 | 2.07 | 2.9E+05 | 1.9E+05 | 1.5E+05 | 3.7E+04 | 8.0E+03 | — | 1.1E+05 | — |
| 3 | 452 | DD, HIV-1 | mLN | 81.4 | 54.4 | 67.6 | 28.5 | 6.72 | 0.19 | 2.0E+06 | 1.1E+06 | 7.2E+05 | 3.0E+05 | 7.2E+04 | — | 7.6E+03 | — |
| 3 | 459 | DD, HIV-1 | mLN | 91.5 | 51.4 | 81.6 | 14.8 | 2.27 | 0.34 | 1.0E+06 | 5.3E+05 | 4.3E+05 | 7.9E+04 | 1.2E+04 | — | ND | — |
DD indicates denileukin diftitox; ND, not detectable; and —, not done.
*
Percentage of human CD45+ of total cells.
†
Percentage of CD3+ from human CD45+ cells.
‡
Percentage of CD4+ or CD8+ from CD45+ CD3+ cells.
§
Percentage of Foxp3+ or p24+ from CD45+CD3+CD8− cells.
‖
Days after HIV-1 infection.