Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1984 May;50(2):615–618. doi: 10.1128/jvi.50.2.615-618.1984

Physical maps of varicella-zoster virus DNA derived with 11 restriction enzymes.

L Mishra, D E Dohner, W J Wellinghoff, L D Gelb
PMCID: PMC255684  PMID: 6323760

Abstract

Varicella-zoster virus DNA was digested with 11 restriction endonucleases, and the resulting fragments were separated on agarose gels. Terminal fragments were identified by lambda exonuclease digestion. Physical maps were then constructed using a combination of double restriction enzyme digestion and hybridization to cloned BamHI fragments to place the remaining fragments in order.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atherton S. S., Sullivan D. C., Dauenhauer S. A., Ruyechan W. T., O'Callaghan D. J. Properties of the genome of equine herpesvirus type 3. Virology. 1982 Jul 15;120(1):18–32. doi: 10.1016/0042-6822(82)90003-4. [DOI] [PubMed] [Google Scholar]
  2. Ben-Porat T., Rixon F. J., Blankenship M. L. Analysis of the structure of the genome of pseudorabies virus. Virology. 1979 Jun;95(2):285–294. doi: 10.1016/0042-6822(79)90484-7. [DOI] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  4. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  5. Davison A. J., Scott J. E. Molecular cloning of the varicella-zoster virus genome and derivation of six restriction endonuclease maps. J Gen Virol. 1983 Aug;64(Pt 8):1811–1814. doi: 10.1099/0022-1317-64-8-1811. [DOI] [PubMed] [Google Scholar]
  6. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  7. Dugaiczyk A., Boyer H. W., Goodman H. M. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975 Jul 25;96(1):171–184. doi: 10.1016/0022-2836(75)90189-8. [DOI] [PubMed] [Google Scholar]
  8. Dumas A. M., Geelen J. L., Weststrate M. W., Wertheim P., van der Noordaa J. XbaI, PstI, and BglII restriction enzyme maps of the two orientations of the varicella-zoster virus genome. J Virol. 1981 Aug;39(2):390–400. doi: 10.1128/jvi.39.2.390-400.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ecker J. R., Hyman R. W. Varicella zoster virus DNA exists as two isomers. Proc Natl Acad Sci U S A. 1982 Jan;79(1):156–160. doi: 10.1073/pnas.79.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gilden D. H., Shtram Y., Friedmann A., Wellish M., Devlin M., Fraser N., Becker Y. The internal organization of the varicella-zoster virus genome. J Gen Virol. 1982 Jun;60(Pt 2):371–374. doi: 10.1099/0022-1317-60-2-371. [DOI] [PubMed] [Google Scholar]
  11. Hayward G. S., Jacob R. J., Wadsworth S. C., Roizman B. Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4243–4247. doi: 10.1073/pnas.72.11.4243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henry B. E., Robinson R. A., Dauenhauer S. A., Atherton S. S., Hayward G. S., O'Callaghan D. J. Structure of the genome of equine herpesvirus type 1. Virology. 1981 Nov;115(1):97–114. doi: 10.1016/0042-6822(81)90092-1. [DOI] [PubMed] [Google Scholar]
  13. Martin J. H., Dohner D. E., Wellinghoff W. J., Gelb L. D. Restriction endonuclease analysis of varicella-zoster vaccine virus and wild-type DNAs. J Med Virol. 1982;9(1):69–76. doi: 10.1002/jmv.1890090110. [DOI] [PubMed] [Google Scholar]
  14. Richards J. C., Hyman R. W., Rapp F. Analysis of the DNAs from seven varicella-zoster virus isolates. J Virol. 1979 Dec;32(3):812–821. doi: 10.1128/jvi.32.3.812-821.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  16. Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol. 1979;68:152–176. doi: 10.1016/0076-6879(79)68011-4. [DOI] [PubMed] [Google Scholar]
  17. Stevely W. S. Inverted repetition in the chromosome of pseudorabies virus. J Virol. 1977 Apr;22(1):232–234. doi: 10.1128/jvi.22.1.232-234.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Straus S. E., Aulakh H. S., Ruyechan W. T., Hay J., Casey T. A., Vande Woude G. F., Owens J., Smith H. A. Structure of varicella-zoster virus DNA. J Virol. 1981 Nov;40(2):516–525. doi: 10.1128/jvi.40.2.516-525.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Straus S. E., Hay J., Smith H., Owens J. Genome differences among varicella-zoster virus isolates. J Gen Virol. 1983 May;64(Pt 5):1031–1041. doi: 10.1099/0022-1317-64-5-1031. [DOI] [PubMed] [Google Scholar]
  20. Straus S. E., Owens J., Ruyechan W. T., Takiff H. E., Casey T. A., Vande Woude G. F., Hay J. Molecular cloning and physical mapping of varicella-zoster virus DNA. Proc Natl Acad Sci U S A. 1982 Feb;79(4):993–997. doi: 10.1073/pnas.79.4.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Swanstrom R., Shank P. R. X-Ray Intensifying Screens Greatly Enhance the Detection by Autoradiography of the Radioactive Isotopes 32P and 125I. Anal Biochem. 1978 May;86(1):184–192. doi: 10.1016/0003-2697(78)90333-0. [DOI] [PubMed] [Google Scholar]
  22. Yang R., Lis J., Wu R. Elution of DNA from agarose gels after electrophoresis. Methods Enzymol. 1979;68:176–182. doi: 10.1016/0076-6879(79)68012-6. [DOI] [PubMed] [Google Scholar]
  23. Zweerink H. J., Morton D. H., Stanton L. W., Neff B. J. Restriction endonuclease analysis of the DNA from varicella-zoster virus: stability of the DNA after passage in vitro. J Gen Virol. 1981 Jul;55(Pt 1):207–211. doi: 10.1099/0022-1317-55-1-207. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES