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. 1984 May;50(2):619–622. doi: 10.1128/jvi.50.2.619-622.1984

Gamma-T4 hybrid bacteriophage carrying the thymidine kinase gene of bacteriophage T4.

A J Mileham, N E Murray, H R Revel
PMCID: PMC255686  PMID: 6323761

Abstract

Among 32 lambda-T4 recombinant phages selected for growth on a thymidylate synthetase-deficient (thyA) host, 2 were shown to carry the T4 thymidine kinase (tk) gene. The lambda-T4tk phages contain two T4 HindIII DNA fragments (2.0 and 1.5 kilobases) that hybridize to restriction fragments of T4 DNA, encompassing the tk locus at 60 kilobases on the T4 map. The T4tk insert compensates for the simultaneous host deficiencies of thymidine kinase and thymidylate synthetase in a thymidine kinase-deficient (tdk) host growing in the presence of fluorodeoxyuridine when provided with thymidine and uridine. The lambda-T4tk hybrid phages specified five polypeptides with Mrs of 22,000 (22K), 21K, 14K, 11K, and 9K.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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