Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1984 May;50(2):636–640. doi: 10.1128/jvi.50.2.636-640.1984

Differential phosphorylation of cytoplasmic and nuclear variants of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses.

K H Scheidtmann, J Schickedanz, G Walter, R E Lanford, J S Butel
PMCID: PMC255692  PMID: 6323765

Abstract

The phosphorylation patterns of cytoplasmic and nuclear forms of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses were analyzed by two-dimensional peptide mapping. The PARA(cT) mutant which encodes a large T antigen defective for nuclear transport was used as source for cytoplasmic large T antigen. The data suggest that the large T antigen is phosphorylated in a sequential manner at a subset of sites in the cytoplasm and at additional sites in the nucleus.

Full text

PDF
636

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumann E. A., Hand R. Phosphorylation and dephosphorylation alter the structure of D2 hybrid T antigen. J Virol. 1982 Oct;44(1):78–87. doi: 10.1128/jvi.44.1.78-87.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ben-Ze'ev A., Duerr A., Solomon F., Penman S. The outer boundary of the cytoskeleton: a lamina derived from plasma membrane proteins. Cell. 1979 Aug;17(4):859–865. doi: 10.1016/0092-8674(79)90326-x. [DOI] [PubMed] [Google Scholar]
  3. Deppert W., Henning R. SV40 T-antigen-related molecules on the surfaces of HeLa cells infected with adenovirus-2-SV40 hybrids and on SV40-transformed cells. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):225–234. doi: 10.1101/sqb.1980.044.01.026. [DOI] [PubMed] [Google Scholar]
  4. Deppert W., Pates R. Cell surface location of simian virus 40-specific proteins on HeLa cells infected with adenovirus type 2-simian virus 40 hybrid viruses Ad2+ND1 and Ad2+ND2. J Virol. 1979 Aug;31(2):522–536. doi: 10.1128/jvi.31.2.522-536.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deppert W., Walter G. Domains of simian virus 40 large T-antigen exposed on the cell surface. Virology. 1982 Oct 15;122(1):56–70. doi: 10.1016/0042-6822(82)90377-4. [DOI] [PubMed] [Google Scholar]
  6. Edwards C. A., Khoury G., Martin R. G. Phosphorylation of T-antigen and control T-antigen expression in cells transformed by wild-type and tsA mutants of simian virus 40. J Virol. 1979 Feb;29(2):753–762. doi: 10.1128/jvi.29.2.753-762.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fanning E., Nowak B., Burger C. Detection and characterization of multiple forms of simian virus 40 large T antigen. J Virol. 1981 Jan;37(1):92–102. doi: 10.1128/jvi.37.1.92-102.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fanning E., Westphal K. H., Brauer D., Cörlin D. Subclasses of simian virus 40 large T antigen: differential binding of two subclasses of T antigen from productively infected cells to viral and cellular DNA. EMBO J. 1982;1(9):1023–1028. doi: 10.1002/j.1460-2075.1982.tb01290.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenspan D. S., Carroll R. B. Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen. Proc Natl Acad Sci U S A. 1981 Jan;78(1):105–109. doi: 10.1073/pnas.78.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klockmann U., Deppert W. Acylation: a new post-translational modification specific for plasma membrane-associated simian virus 40 large T-antigen. FEBS Lett. 1983 Jan 24;151(2):257–259. doi: 10.1016/0014-5793(83)80081-7. [DOI] [PubMed] [Google Scholar]
  11. Kress M., Resche-Rigon M., Feunteun J. Phosphorylation pattern of large T antigens in mouse cells infected by simian virus 40 wild type or deletion mutants. J Virol. 1982 Sep;43(3):761–771. doi: 10.1128/jvi.43.3.761-771.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lanford R. E., Butel J. S. Antigenic relationship of SV40 early proteins to purified large T polypeptide. Virology. 1979 Sep;97(2):295–306. doi: 10.1016/0042-6822(79)90341-6. [DOI] [PubMed] [Google Scholar]
  13. Lanford R. E., Butel J. S. Biochemical characterization of nuclear and cytoplasmic forms of SV40 tumor antigens encoded by parental and transport-detective mutant SV40-adenovirus 7 hybrid viruses. Virology. 1980 Sep;105(2):314–327. doi: 10.1016/0042-6822(80)90033-1. [DOI] [PubMed] [Google Scholar]
  14. Lanford R. E., Butel J. S. Inhibition of nuclear migration of wild-type SV40 tumor antigen by a transport-defective mutant of SV40-adenovirus 7 hybrid virus. Virology. 1980 Sep;105(2):303–313. doi: 10.1016/0042-6822(80)90032-x. [DOI] [PubMed] [Google Scholar]
  15. Mann K., Hunter T., Walter G., Linke H. Evidence for simian virus 40 (SV40) coding of SV40 T-antigen and the SV40-specific proteins in HeLa cells infected with nondefective adenovirus type 2-SV40 hybrid viruses. J Virol. 1977 Oct;24(1):151–169. doi: 10.1128/jvi.24.1.151-169.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Montenarh M., Henning R. Self-assembly of simian virus 40 large T antigen oligomers by divalent cations. J Virol. 1983 Feb;45(2):531–538. doi: 10.1128/jvi.45.2.531-538.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morrison B., Kress M., Khoury G., Jay G. Simian virus 40 tumor antigen: isolation of the origin-specific DNA-binding domain. J Virol. 1983 Jul;47(1):106–114. doi: 10.1128/jvi.47.1.106-114.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Parker B. A., Stark G. R. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA. J Virol. 1979 Aug;31(2):360–369. doi: 10.1128/jvi.31.2.360-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Santos M., Butel J. S. Association of SV40 large tumor antigen and cellular proteins on the surface of SV40-transformed mouse cells. Virology. 1982 Jul 15;120(1):1–17. doi: 10.1016/0042-6822(82)90002-2. [DOI] [PubMed] [Google Scholar]
  20. Scheidtmann K. H., Echle B., Walter G. Simian virus 40 large T antigen is phosphorylated at multiple sites clustered in two separate regions. J Virol. 1982 Oct;44(1):116–133. doi: 10.1128/jvi.44.1.116-133.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scheidtmann K. H., Hardung M., Echle B., Walter G. DNA-binding activity of simian virus 40 large T antigen correlates with a distinct phosphorylation state. J Virol. 1984 Apr;50(1):1–12. doi: 10.1128/jvi.50.1.1-12.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scheidtmann K. H., Kaiser A., Carbone A., Walter G. Phosphorylation of threonine in the proline-rich carboxy-terminal region of simian virus 40 large T antigen. J Virol. 1981 Apr;38(1):59–69. doi: 10.1128/jvi.38.1.59-69.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Soule H. R., Lanford R. E., Butel J. S. Antigenic and immunogenic characteristics of nuclear and membrane-associated simian virus 40 tumor antigen. J Virol. 1980 Feb;33(2):887–901. doi: 10.1128/jvi.33.2.887-901.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tegtmeyer P., Schwartz M., Collins J. K., Rundell K. Regulation of tumor antigen synthesis by simain virus 40 gene A. J Virol. 1975 Jul;16(1):168–178. doi: 10.1128/jvi.16.1.168-178.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tjian R. Regulation of viral transcription and DNA replication by the SV40 large T antigen. Curr Top Microbiol Immunol. 1981;93:5–24. doi: 10.1007/978-3-642-68123-3_2. [DOI] [PubMed] [Google Scholar]
  26. Van Roy F., Fransen L., Fiers W. Metabolic turnover of phosphorylation sites in simian virus 40 large T antigen. J Virol. 1983 Jan;45(1):442–446. doi: 10.1128/jvi.45.1.442-446.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. van Roy F., Fransen L., Fiers W. Improved localization of phosphorylation sites in simian virus 40 large T antigen. J Virol. 1983 Jan;45(1):315–331. doi: 10.1128/jvi.45.1.315-331.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES