Isolation of Genomic DNA from Mouse Tails

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Protocol

• Cut tail pieces (3mm) and mark ears.

• Add 720 ml STE and 30 ml Proteinase K (10 mg/ml stock).

• Incubate at 55°C on heating block and vortex every hour for 3 hours at top speed for 10 seconds.

• Inactivate proteinase K at 70°C for 5 minutes.

• Quench on ice for 5 minutes.

• Centrifuge tail DNA for 10 minutes at full speed.

• Decant into new tube containing 720 ml Isopropanol. 

• Precipitate DNA by inverting the tube or vortexing.

• Spin down DNA for 5 minutes at full speed. Remove supernatant.

• Wash pellet with 70% ethanol.

• Spin down genomic DNA 5 minutes at full speed. Remove supernatant.

• Allow DNA to dry for 1-2 minutes.

• Resuspend DNA in 100-200 ml depending on size of pellet.

• Place tube at 55°C for 1 hour to facilitate dissolution of DNA.