Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1987 Nov;61(11):3645–3647. doi: 10.1128/jvi.61.11.3645-3647.1987

Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein.

R Ostermayr 1, K von der Helm 1, V Gauss-Müller 1, E L Winnacker 1, F Deinhardt 1
PMCID: PMC255970  PMID: 2822970

Abstract

The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with rabbit anti-HAV serum, suggesting that it possesses HAV antigenicity.

Full text

PDF
3645

Images in this article

3647Image
on p.3647

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baroudy B. M., Ticehurst J. R., Miele T. A., Maizel J. V., Jr, Purcell R. H., Feinstone S. M. Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2143–2147. doi: 10.1073/pnas.82.7.2143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coulepis A. G., Tannock G. A., Locarnini S. A., Gust I. D. Evidence that the genome of hepatitis A virus consists of single-stranded RNA. J Virol. 1981 Jan;37(1):473–477. doi: 10.1128/jvi.37.1.473-477.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Emini E. A., Hughes J. V., Perlow D. S., Boger J. Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. J Virol. 1985 Sep;55(3):836–839. doi: 10.1128/jvi.55.3.836-839.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frösner G. G., Deinhardt F., Scheid R., Gauss-Müller V., Holmes N., Messelberger V., Siegl G., Alexander J. J. Propagation of human hepatitis A virus in a hepatoma cell line. Infection. 1979;7(6):303–305. doi: 10.1007/BF01642154. [DOI] [PubMed] [Google Scholar]
  5. Gauss-Müller V., Frösner G. G., Deinhardt F. Propagation of hepatitis A virus in human embryo fibroblasts. J Med Virol. 1981;7(3):233–239. doi: 10.1002/jmv.1890070308. [DOI] [PubMed] [Google Scholar]
  6. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hughes J. V., Stanton L. W. Isolation and immunizations with hepatitis A viral structural proteins: induction of antiprotein, antiviral, and neutralizing responses. J Virol. 1985 Aug;55(2):395–401. doi: 10.1128/jvi.55.2.395-401.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hughes J. V., Stanton L. W., Tomassini J. E., Long W. J., Scolnick E. M. Neutralizing monoclonal antibodies to hepatitis A virus: partial localization of a neutralizing antigenic site. J Virol. 1984 Nov;52(2):465–473. doi: 10.1128/jvi.52.2.465-473.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Linemeyer D. L., Menke J. G., Martin-Gallardo A., Hughes J. V., Young A., Mitra S. W. Molecular cloning and partial sequencing of hepatitis A viral cDNA. J Virol. 1985 May;54(2):247–255. doi: 10.1128/jvi.54.2.247-255.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Locarnini S. A., Coulepis A. G., Westaway E. G., Gust I. D. Restricted replication of human hepatitis A virus in cell culture: intracellular biochemical studies. J Virol. 1981 Jan;37(1):216–225. doi: 10.1128/jvi.37.1.216-225.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Melnick J. L. Classification of hepatitis A virus as enterovirus type 72 and of hepatitis B virus as hepadnavirus type 1. Intervirology. 1982;18(3):105–106. doi: 10.1159/000149313. [DOI] [PubMed] [Google Scholar]
  12. Najarian R., Caput D., Gee W., Potter S. J., Renard A., Merryweather J., Van Nest G., Dina D. Primary structure and gene organization of human hepatitis A virus. Proc Natl Acad Sci U S A. 1985 May;82(9):2627–2631. doi: 10.1073/pnas.82.9.2627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Provost P. J., Hilleman M. R. Propagation of human hepatitis A virus in cell culture in vitro. Proc Soc Exp Biol Med. 1979 Feb;160(2):213–221. doi: 10.3181/00379727-160-40422. [DOI] [PubMed] [Google Scholar]
  14. Rüther U., Müller-Hill B. Easy identification of cDNA clones. EMBO J. 1983;2(10):1791–1794. doi: 10.1002/j.1460-2075.1983.tb01659.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Siegl G., Frösner G. G. Characterization and classification of virus particles associated with hepatitis A. II. Type and configuration of nucleic acid. J Virol. 1978 Apr;26(1):48–53. doi: 10.1128/jvi.26.1.48-53.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ticehurst J. R., Racaniello V. R., Baroudy B. M., Baltimore D., Purcell R. H., Feinstone S. M. Molecular cloning and characterization of hepatitis A virus cDNA. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5885–5889. doi: 10.1073/pnas.80.19.5885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. von der Helm K., Winnacker E. L., Deinhardt F., Frösner G., Gauss-Müller V., Bayerl B., Scheid R., Siegl G. Cloning of hepatitis A virus genome. J Virol Methods. 1981 Jul;3(1):37–43. doi: 10.1016/0166-0934(81)90020-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES