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. 1987 Dec;61(12):3983–3991. doi: 10.1128/jvi.61.12.3983-3991.1987

Effect of NH4+ ions on phi 29 DNA-protein p3 replication: formation of a complex between the terminal protein and the DNA polymerase.

L Blanco 1, I Prieto 1, J Gutiérrez 1, A Bernad 1, J M Lázaro 1, J M Hermoso 1, M Salas 1
PMCID: PMC256019  PMID: 3682063

Abstract

Ammonium ions stimulated the formation of the phi diameter 29 protein p3-dAMP initiation complex by decreasing the Km value for dATP in a purified system containing the viral terminal protein p3, the viral DNA polymerase p2, and the phi 29 DNA-protein p3 complex as a template. In addition, NH4+ ions stimulated the amount of p3-dAMP complex elongation and increased by about twofold the rate of elongation. The stimulatory effect of NH4+ ions on in vitro phi 29 DNA replication is probably related to the formation of a stable complex between the terminal protein and the DNA polymerase, which was detected only in the presence of NH4+ ions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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