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. 1987 Dec;61(12):4030–4032. doi: 10.1128/jvi.61.12.4030-4032.1987

Role of a structural glycoprotein of pseudorabies in virus virulence.

T C Mettenleiter 1, L Zsak 1, A S Kaplan 1, T Ben-Porat 1, B Lomniczi 1
PMCID: PMC256027  PMID: 2824832

Abstract

The virulence of deletion mutants of pseudorabies virus defective in the expression of glycoprotein gI, gp63, or both was tested in 1-day-old chickens and young pigs. In the absence of expression of gI, the virulence of a fully virulent laboratory strain, PrV(Ka), for 1-day-old chickens was reduced approximately fourfold. Inactivation of glycoprotein gp63 appeared also to affect the virulence of PrV(Ka) only slightly, as did inactivation of both gI and gp63. The level of reduction in virulence, however, was considerably more marked in Bartha 43/25aB4, a less virulent virus strain. Inactivation of the expression of gI in Bartha 43/25aB4 reduced virulence for chickens at least 100-fold. The results obtained when the virulence of the mutants for pigs was determined were compatible with those obtained for chickens. These results indicate that gI plays a role in virulence, but that it does so in conjunction with at least one other viral function (a function that is defective in Bartha 43/25aB4).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berns A., van den Ouweland A., Quint W., van Oirschot J., Gielkens A. Presence of markers for virulence in the unique short region or repeat region or both of pseudorabies hybrid viruses. J Virol. 1985 Jan;53(1):89–93. doi: 10.1128/jvi.53.1.89-93.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gielkens A. L., Van Oirschot J. T., Berns A. J. Genome differences among field isolates and vaccine strains of pseudorabies virus. J Gen Virol. 1985 Jan;66(Pt 1):69–82. doi: 10.1099/0022-1317-66-1-69. [DOI] [PubMed] [Google Scholar]
  3. Kit S., Kit M., Pirtle E. C. Attenuated properties of thymidine kinase-negative deletion mutant of pseudorabies virus. Am J Vet Res. 1985 Jun;46(6):1359–1367. [PubMed] [Google Scholar]
  4. Klein R. J. The pathogenesis of acute, latent and recurrent herpes simplex virus infections. Arch Virol. 1982;72(3):143–168. doi: 10.1007/BF01348961. [DOI] [PubMed] [Google Scholar]
  5. Kümel G., Kaerner H. C., Levine M., Schröder C. H., Glorioso J. C. Passive immune protection by herpes simplex virus-specific monoclonal antibodies and monoclonal antibody-resistant mutants altered in pathogenicity. J Virol. 1985 Dec;56(3):930–937. doi: 10.1128/jvi.56.3.930-937.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lomniczi B., Blankenship M. L., Ben-Porat T. Deletions in the genomes of pseudorabies virus vaccine strains and existence of four isomers of the genomes. J Virol. 1984 Mar;49(3):970–979. doi: 10.1128/jvi.49.3.970-979.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lomniczi B., Watanabe S., Ben-Porat T., Kaplan A. S. Genetic basis of the neurovirulence of pseudorabies virus. J Virol. 1984 Oct;52(1):198–205. doi: 10.1128/jvi.52.1.198-205.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lomniczi B., Watanabe S., Ben-Porat T., Kaplan A. S. Genome location and identification of functions defective in the Bartha vaccine strain of pseudorabies virus. J Virol. 1987 Mar;61(3):796–801. doi: 10.1128/jvi.61.3.796-801.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGregor S., Easterday B. C., Kaplan A. S., Ben-Porat T. Vaccination of swine with thymidine kinase-deficient mutants of pseudorabies virus. Am J Vet Res. 1985 Jul;46(7):1494–1497. [PubMed] [Google Scholar]
  10. Mettenleiter T. C., Lukàcs N., Rziha H. J. Pseudorabies virus avirulent strains fail to express a major glycoprotein. J Virol. 1985 Oct;56(1):307–311. doi: 10.1128/jvi.56.1.307-311.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mettenleiter T. C., Schreurs C., Zuckermann F., Ben-Porat T. Role of pseudorabies virus glycoprotein gI in virus release from infected cells. J Virol. 1987 Sep;61(9):2764–2769. doi: 10.1128/jvi.61.9.2764-2769.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Petrovskis E. A., Timmins J. G., Gierman T. M., Post L. E. Deletions in vaccine strains of pseudorabies virus and their effect on synthesis of glycoprotein gp63. J Virol. 1986 Dec;60(3):1166–1169. doi: 10.1128/jvi.60.3.1166-1169.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Petrovskis E. A., Timmins J. G., Post L. E. Use of lambda gt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins. J Virol. 1986 Oct;60(1):185–193. doi: 10.1128/jvi.60.1.185-193.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Quint W., Gielkens A., Van Oirschot J., Berns A., Cuypers H. T. Construction and characterization of deletion mutants of pseudorabies virus: a new generation of 'live' vaccines. J Gen Virol. 1987 Feb;68(Pt 2):523–534. doi: 10.1099/0022-1317-68-2-523. [DOI] [PubMed] [Google Scholar]
  15. Tenser R. B., Ressel S. J., Fralish F. A., Jones J. C. The role of pseudorabies virus thymidine kinase expression in trigeminal ganglion infection. J Gen Virol. 1983 Jun;64(Pt 6):1369–1373. doi: 10.1099/0022-1317-64-6-1369. [DOI] [PubMed] [Google Scholar]

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