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. 1982 Apr;42(1):100–105. doi: 10.1128/jvi.42.1.100-105.1982

Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA.

C Pourcel, A Louise, M Gervais, N Chenciner, M F Dubois, P Tiollais
PMCID: PMC256049  PMID: 7086959

Abstract

Mouse L cells transformed with recombinant plasmids carrying hepatitis B virus (HBV) DNA fragments were used to study the transcription of the viral surface antigen gene (gene S). An HBV-specific, polyadenylated, 2.3-kilobase RNA was mapped on the HBV genome. This RNA hybridized with approximately 75% of the genome and excluded the region of the HBV core antigen gene (gene C). The 2.3-kilobase RNA species was present only in cell lines that produced hepatitis B surface antigen. An HBV-specific 2.3-kilobase RNA was also detected in human hepatoma cell line PLC/PRF/5 which produced hepatitis B surface antigen. A study of gene S expression in the transformed mouse L cells allowed us to localize the regions of initiation and termination of gene S transcription. Our results strongly suggest that the 2.3-kilobase RNA molecule is the mRNA of the major polypeptide of the envelope, which carries the viral surface antigen determinants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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