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. 1982 Apr;42(1):165–175. doi: 10.1128/jvi.42.1.165-175.1982

Continuing germ line integration of AKV proviruses during the breeding of AKR mice and derivative recombinant inbred strains.

D L Steffen, B A Taylor, R A Weinberg
PMCID: PMC256057  PMID: 6283136

Abstract

The gel electrophoresis-hybridization technique of Southern was used to analyze genetically transmitted proviruses coding for the AKV strain of murine leukemia virus. We were able to identify the restriction endonuclease EcoRI fragments containing two previously unidentified, genetically transmitted AKV proviruses of AKR mice. Comparison of different sublines of AKR mice revealed considerable heterogeneity in their complement of germ line proviruses. This heterogeneity provides evidence that the provirus complement of AKR mice is not stable. Rather, the number of genetically transmitted proviruses increases during inbreeding. Examination of a series of sublines of the C3H strain indicated that this amplification is dependent on viremia. We estimate that, in viremic strains of mice, one new provirus becomes fixed in the germ line every 15 to 30 years.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Stephenson J. R. Endogenous type-C RNA viruses of mammalian cells. Biochim Biophys Acta. 1976 Dec 23;458(4):323–354. doi: 10.1016/0304-419x(76)90006-8. [DOI] [PubMed] [Google Scholar]
  2. Chattopadhyay S. K., Lander M. R., Rands E., Lowy D. R. Structure of endogenous murine leukemia virus DNA in mouse genomes. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5774–5778. doi: 10.1073/pnas.77.10.5774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chattopadhyay S. K., Lowy D. R., Teich N. M., Levine A. S., Rowe W. P. Qualitative and quantitative studies of AKR-type murine leukemia virus sequences in mouse DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1085–1101. doi: 10.1101/sqb.1974.039.01.124. [DOI] [PubMed] [Google Scholar]
  4. Chattopadhyay S. K., Rowe W. P., Teich N. M., Lowy D. R. Definitive evidence that the murine C-type virus inducing locus Akv-1 is viral genetic material. Proc Natl Acad Sci U S A. 1975 Mar;72(3):906–910. doi: 10.1073/pnas.72.3.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hughes S. H., Vogt P. K., Stubblefield E., Robinson H., Bishop J. M., Varmus H. E. Organization of endogenous and exogenous viral and linked nonviral sequences. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):1077–1089. doi: 10.1101/sqb.1980.044.01.116. [DOI] [PubMed] [Google Scholar]
  6. Ihle J. N., Joseph D. R. Genetic analysis of the endogenous C3H murine leukemia virus genome: evidence for one locus unlinked to the endogenous murine leukemia virus genome of C57BL/6 mice. Virology. 1978 Jun 15;87(2):298–306. doi: 10.1016/0042-6822(78)90135-6. [DOI] [PubMed] [Google Scholar]
  7. Ihle J. N., Joseph D. R. Serological and virological analysis of NIH (NIH X AKR) mice: evidence for three AKR murine leukemia virus loci. Virology. 1978 Jun 15;87(2):287–297. doi: 10.1016/0042-6822(78)90134-4. [DOI] [PubMed] [Google Scholar]
  8. Jaenisch R. Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1260–1264. doi: 10.1073/pnas.73.4.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kozak C. A., Rowe W. P. Genetic mapping of the ecotropic virus-inducing locus Akv-2 of the AKR mouse. J Exp Med. 1980 Nov 1;152(5):1419–1423. doi: 10.1084/jem.152.5.1419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kuff E. L., Lueders K. K., Scolnick E. M. Nucleotide sequence relationship between intracisternal type A particles of Mus musculus and an endogenous retrovirus (M432) of Mus cervicolor. J Virol. 1978 Oct;28(1):66–74. doi: 10.1128/jvi.28.1.66-74.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LYNCH C. J. The R.I.L. strain of mice: its relation to the leukemic AK strain and AKR substrains. J Natl Cancer Inst. 1954 Aug;15(1):161–176. [PubMed] [Google Scholar]
  12. Léonard A., Deknudt G. A new marker for chromosome studies in the mouse. Nature. 1967 Apr 29;214(5087):504–505. doi: 10.1038/214504a0. [DOI] [PubMed] [Google Scholar]
  13. Quint W., Quax W., van der Putten H., Berns A. Characterization of AKR murine leukemia virus sequences in AKR mouse substrains and structure of integrated recombinant genomes in tumor tissues. J Virol. 1981 Jul;39(1):1–10. doi: 10.1128/jvi.39.1.1-10.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rowe W. P., Hartley J. W., Bremner T. Genetic mapping of a murine leukemia virus-inducing locus of AKR mice. Science. 1972 Nov 24;178(4063):860–862. doi: 10.1126/science.178.4063.860. [DOI] [PubMed] [Google Scholar]
  15. Rowe W. P., Kozak C. A. Germ-line reinsertions of AKR murine leukemia virus genomes in Akv-1 congenic mice. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4871–4874. doi: 10.1073/pnas.77.8.4871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rowe W. P. Studies of genetic transmission of murine leukemia virus by AKR mice. I. Crosses with Fv-1 n strains of mice. J Exp Med. 1972 Nov 1;136(5):1272–1285. doi: 10.1084/jem.136.5.1272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. Staats J. Standardized nomenclature for inbred strains of mice: sixth listing. Cancer Res. 1976 Dec;36(12):4333–4377. [PubMed] [Google Scholar]
  19. Steffen D. L., Bird S., Weinberg R. A. Evidence for the Asiatic origin of endogenous AKR-type murine leukemia proviruses. J Virol. 1980 Sep;35(3):824–835. doi: 10.1128/jvi.35.3.824-835.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Steffen D., Bird S., Rowe W. P., Weinberg R. A. Identification of DNA fragments carrying ecotropic proviruses of AKR mice. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4554–4558. doi: 10.1073/pnas.76.9.4554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stockert E., DeLeo A. B., O'Donnell P. V., Obata Y., Old L. J. G(AKSL2): a new cell surface antigen of the mouse related to the dualtropic mink cell focus-inducing class of murine leukemia virus detected by naturally occurring antibody. J Exp Med. 1979 Jan 1;149(1):200–215. doi: 10.1084/jem.149.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Taylor B. A., Meier H. Mapping the adrenal lipid depletion gene of the AKR/J mouse strain. Genet Res. 1975 Dec;26(3):307–312. doi: 10.1017/s0016672300016104. [DOI] [PubMed] [Google Scholar]

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