Abstract
Nonlimit chemical cleavage at tryptophan residues of protein labeled at the amino terminus afforded a simple procedure for generating specific fragments and for mapping tryptophan positions. A comparison of the matrix (M) and nucleocapsid (N) proteins of four members of the Vesiculovirus group by this procedure suggests considerable conservation of tryptophan number and location in the four serotypes examined.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bonner W. M., Stedman J. D. Efficient fluorography of 3H and 14C on thin layers. Anal Biochem. 1978 Aug 15;89(1):247–256. doi: 10.1016/0003-2697(78)90747-9. [DOI] [PubMed] [Google Scholar]
- Brown E. G., Prevec L. Comparative analyses of vesiculovirus proteins utilizing partial cleavage fragments at tryptophan residues. Virology. 1979 May;95(1):244–248. doi: 10.1016/0042-6822(79)90424-0. [DOI] [PubMed] [Google Scholar]
- Brown E., Prevec L. Proteins of vesicular stomatitis virus. IV. A comparison of tryptic peptides of the vesicular stomatitis group of rhabdoviruses. Virology. 1978 Aug;89(1):7–21. doi: 10.1016/0042-6822(78)90035-1. [DOI] [PubMed] [Google Scholar]
- Garoff H., Söderlund H. The amphiphilic membrane glycoproteins of Semliki Forest virus are attached to the lipid bilayer by their COOH-terminal ends. J Mol Biol. 1978 Sep 25;124(3):535–549. doi: 10.1016/0022-2836(78)90186-9. [DOI] [PubMed] [Google Scholar]
- Ghosh H. P., Toneguzzo F., Wells S. Synthesis in vitro of vesicular stomatitis virus proteins in cytoplasmic extracts of L cells. Biochem Biophys Res Commun. 1973 Sep 5;54(1):228–233. doi: 10.1016/0006-291x(73)90912-1. [DOI] [PubMed] [Google Scholar]
- Ghosh K., Ghosh H. P., Simsek M., Raj Bhandary U. L. Initiator methionine transfer ribonucleic acid from wheat embryo. Purification, properties, and partial nucleotide sequences. J Biol Chem. 1974 Aug 10;249(15):4720–4729. [PubMed] [Google Scholar]
- Gillam I., Blew D., Warrington R. C., von Tigerstrom M., Tener G. M. A general procedure for the isolation of specific transfer ribonucleic acids. Biochemistry. 1968 Oct;7(10):3459–3468. doi: 10.1021/bi00850a022. [DOI] [PubMed] [Google Scholar]
- Nikodem V., Fresco J. R. Protein fingerprinting by SDS-gel electrophoresis after partial fragmentation with CNBr. Anal Biochem. 1979 Sep 1;97(2):382–386. doi: 10.1016/0003-2697(79)90089-7. [DOI] [PubMed] [Google Scholar]
- Pringle C. R., Duncan I. B., Stevenson M. Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype. J Virol. 1971 Dec;8(6):836–841. doi: 10.1128/jvi.8.6.836-841.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shechter Y., Patchornik A., Burstein Y. Selective chemical cleavage of tryptophanyl peptide bonds by oxidative chlorination with N-chlorosuccinimide. Biochemistry. 1976 Nov 16;15(23):5071–5075. doi: 10.1021/bi00668a019. [DOI] [PubMed] [Google Scholar]
- Smith A. E., Marcker K. A. Cytoplasmic methionine transfer RNAs from eukaryotes. Nature. 1970 May 16;226(5246):607–610. doi: 10.1038/226607a0. [DOI] [PubMed] [Google Scholar]
- Smith A. E. The initiation of protein synthesis directed by the RNA from encephalomyocarditis virus. Eur J Biochem. 1973 Mar 1;33(2):301–313. doi: 10.1111/j.1432-1033.1973.tb02684.x. [DOI] [PubMed] [Google Scholar]