Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1982 Jul;43(1):158–165. doi: 10.1128/jvi.43.1.158-165.1982

Identification of a unique mouse mammary tumor virus in the BALB/cNIV mouse strain.

J P Puma, T G Fanning, L J Young, R D Cardiff
PMCID: PMC256106  PMID: 6286994

Abstract

We examined the genetic structure, in terms of restriction endonuclease recognition sites, of the milk-transmitted, low-oncogenic mouse mammary tumor virus (MuMTV) of the BALB/cNIV mouse strain. An analysis with EcoRI documented the presence of acquired cNIV proviruses in the mammary tumor DNAs of BALB/cNIV animals. A comparison of tumor DNAs digested with PstI showed that both the cNIV MuMTV and C3Hf MuMTV proviruses lacked the 4.3- and 1.1-kilobase pair fragments characteristic of C3H MuMTV patterns. An examination of mammary tumor and normal, nonmammary tissue DNAs with BamHI supported the idea that the cNIV MuMTV is identical to the C3Hf MuMTV and demonstrated that these two low-oncogenic proviruses are identical to the high-oncogenic C3H MuMTV provirus with respect to a pair of BamHI sites which define a 1.3-kilobase pair fragment. For each of the three MuMTV strains, we also mapped DNAs generated in isolated virions by reverse transcription of their genomic RNAs. Our results showed that cNIV and C3Hf MuMTV are distinct entities by virtue of an additional PstI site within the cNIV long terminal repeat sequence. Another unique feature of cNIV MuMTV revealed by the analysis of virion-generated DNAs was the existence of a family of genomes within the cNIV population. We concluded that cNIV is distinct from its presumptive C3Hf MuMTV predecessor.

Full text

PDF
158

Images in this article

160Image
on p.160
161Image
on p.161
161Image
on p.161
162Image
on p.162
162Image
on p.162

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blair P. B. Immunology of the mouse mammary tumor virus: comparison of the antigenicity of mammary tumor virus obtained from several strains of mice. Cancer Res. 1970 Mar;30(3):625–631. [PubMed] [Google Scholar]
  2. Cohen J. C., Majors J. E., Varmus H. E. Organization of mouse mammary tumor virus-specific DNA endogenous to BALB/c mice. J Virol. 1979 Nov;32(2):483–496. doi: 10.1128/jvi.32.2.483-496.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen J. C., Shank P. R., Morris V. L., Cardiff R., Varmus H. E. Integration of the DNA of mouse mammary tumor virus in virus-infected normal and neoplastic tissue of the mouse. Cell. 1979 Feb;16(2):333–345. doi: 10.1016/0092-8674(79)90010-2. [DOI] [PubMed] [Google Scholar]
  4. Cohen J. C., Varmus H. E. Endogenous mammary tumour virus DNA varies among wild mice and segregates during inbreeding. Nature. 1979 Mar 29;278(5703):418–423. doi: 10.1038/278418a0. [DOI] [PubMed] [Google Scholar]
  5. Cohen J. C., Varmus H. E. Proviruses of mouse mammary tumor virus in normal and neoplastic tissues from GR and C3Hf mouse strains. J Virol. 1980 Aug;35(2):298–305. doi: 10.1128/jvi.35.2.298-305.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dickson C., Peters G. Protein-coding potential of mouse mammary tumor virus genome RNA as examined by in vitro translation. J Virol. 1981 Jan;37(1):36–47. doi: 10.1128/jvi.37.1.36-47.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dickson C., Smith R., Peters G. In vitro synthesis of polypeptides encoded by the long terminal repeat region of mouse mammary tumour virus DNA. Nature. 1981 Jun 11;291(5815):511–513. doi: 10.1038/291511a0. [DOI] [PubMed] [Google Scholar]
  8. Dina D., Benz E. W., Jr Structure of murine sarcoma virus DNA replicative intermediates synthesized in vitro. J Virol. 1980 Jan;33(1):377–389. doi: 10.1128/jvi.33.1.377-389.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Donehower L. A., Huang A. L., Hager G. L. Regulatory and coding potential of the mouse mammary tumor virus long terminal redundancy. J Virol. 1981 Jan;37(1):226–238. doi: 10.1128/jvi.37.1.226-238.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fanning T. G., Puma J. P., Cardiff R. D. Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice. J Virol. 1980 Oct;36(1):109–114. doi: 10.1128/jvi.36.1.109-114.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gilboa E., Goff S., Shields A., Yoshimura F., Mitra S., Baltimore D. In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of Moloney murine leukemia virus. Cell. 1979 Apr;16(4):863–874. doi: 10.1016/0092-8674(79)90101-6. [DOI] [PubMed] [Google Scholar]
  12. Hageman P. C., Links J., Bentvelzen P. Biological properties of B particles from C3H and C3Hf mouse milk. J Natl Cancer Inst. 1968 Jun;40(6):1319–1324. [PubMed] [Google Scholar]
  13. Majors J. E., Varmus H. E. Nucleotide sequences at host-proviral junctions for mouse mammary tumour virus. Nature. 1981 Jan 22;289(5795):253–258. doi: 10.1038/289253a0. [DOI] [PubMed] [Google Scholar]
  14. Massey R. J., Schochetman G. Gene order of mouse mammary tumor virus precusor polyproteins and their interaction leading to the formation of a virus. Virology. 1979 Dec;99(2):358–371. doi: 10.1016/0042-6822(79)90015-1. [DOI] [PubMed] [Google Scholar]
  15. Moore D. H., Holben J. A., Charney J. Biologic characteristics of some mouse mammary tumor viruses. J Natl Cancer Inst. 1976 Oct;57(4):889–896. doi: 10.1093/jnci/57.4.889. [DOI] [PubMed] [Google Scholar]
  16. Moore D. H., Long C. A., Vaidya A. B., Sheffield J. B., Dion A. S., Lasfargues E. Y. Mammary tumor viruses. Adv Cancer Res. 1979;29:347–418. doi: 10.1016/s0065-230x(08)60850-7. [DOI] [PubMed] [Google Scholar]
  17. PITELKA D. R., BERN H. A., NANDI S., DEOME K. B. ON THE SIGNIFICANCE OF VIRUS-LIKE PARTICLES IN MAMMARY TISSUES OF C3HF MICE. J Natl Cancer Inst. 1964 Nov;33:867–885. [PubMed] [Google Scholar]
  18. Rothenberg E., Smotkin D., Baltimore D., Weinberg R. A. In vitro synthesis of infectious DNA of murine leukaemia virus. Nature. 1977 Sep 8;269(5624):122–126. doi: 10.1038/269122a0. [DOI] [PubMed] [Google Scholar]
  19. Shank P. R., Cohen J. C., Varmus H. E., Yamamoto K. R., Ringold G. M. Mapping of linear and circular forms of mouse mammary tumor virus DNA with restriction endonucleases: evidence for a large specific deletion occurring at high frequency during circularization. Proc Natl Acad Sci U S A. 1978 May;75(5):2112–2116. doi: 10.1073/pnas.75.5.2112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vacquier J. P., Cardiff R. D., Blair P. B. Immunological characterization of a low oncogenic mouse mammary tumor virus BALB/cNIV mice. J Virol. 1981 Oct;40(1):56–64. doi: 10.1128/jvi.40.1.56-64.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Varmus H. E., Heasley S., Kung H. J., Oppermann H., Smith V. C., Bishop J. M., Shank P. R. Kinetics of synthesis, structure and purification of avian sarcoma virus-specific DNA made in the cytoplasm of acutely infected cells. J Mol Biol. 1978 Mar 25;120(1):55–82. doi: 10.1016/0022-2836(78)90295-4. [DOI] [PubMed] [Google Scholar]
  22. van Nie R., Verstraeten A. A. Studies of genetic transmission of mammary tumour virus by C3Hf mice. Int J Cancer. 1975 Dec 15;16(6):922–931. doi: 10.1002/ijc.2910160606. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES