Figure 2.

Nessy mice produce lower titres of anti-Salmonella antibodies. Titres of Salmonella enterica serovar Dublin-specific (a) immunoglobulin M (IgM), (b) IgG1, (c) IgG2a and (d) IgG3 from nessy (filled circles) and C57BL/6 (open circles) mice killed at the indicated times after infection. Plasma was obtained from blood taken at the time of death. Results represent titres from five or six mice at each time-point infected in three separate experiments. Data were analysed using a two-tailed Student’s t-test; significant differences are indicated above the corresponding data (*P < 0·05, **P < 0·005). Log 2·0 was the smallest measurable titre; titres at or below the detection limit were given a value of 2·0 for the calculation of means and statistical significance.