Figure 4.

Nessy T cells up-regulate the activation marker CD69 to a lesser extent than wild-type cells. (a) Flow cytometry plots showing typical expression of CD44 on nessy and wild-type CD4 and CD8 T cells. Splenocytes from nessy and wild-type mice were analysed for expression of the cell surface markers CD4, CD8 and CD44 by flow cytometry. Plots on the left show cells from a wild-type mouse after gating on lymphocytes by size and granularity whereas those on the right show cells from a nessy mouse using the same gating. Note that the majority of nessy cells are CD44^hi, while the wild-type cells include both CD44^hi and CD44^lo cells. (b–d) Nessy (Ly5^b) (closed circles) and C57BL/6 (Ly5^a) (open circles) splenocytes were cultured overnight together in a ratio of approximately 4 : 1. Cells were stimulated with anti-TCR-β or lipopolysaccharide (LPS) serially diluted in 1/2 log intervals. Expression of CD69 on CD4⁺ (b), CD8⁺ (c) and B (d) cells was measured by flow cytometry. Mean fluorescence intensity of CD69 is shown. Data shown are from one representative experiment from a total of three; unstim., unstimulated.